fa بررسی ارزش بیومارکری ژن SLC1A2در نمونه های بافت بیماران مبتلا به گلیوبلاستوما مولتی فرم ژنتیک Genetics مقاله پژوهشی Research Article <br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> گلیوبلاستوما مولتی فرم (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">GBM</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آستروسیتوما</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> گرید </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">IV</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 53% کلیه گلیوماها را تشکیل می دهد، شایع ترین و کشنده ترین تومور بدخیم اولیه مغز در بزرگسالان است</span></span><span dir="LTR" style="font-size:10.0pt">.</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> میزان مرگ و میر در گلیوبلاستومامولتی فرم بسیار بالا می باشد. هدف از این مطالعه بررسی بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SLC1A2</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در بافت توموری و حاشیه ای سالم افراد مبتلابه گلیوبلاستوما مولتی فرم است.</span></span><span dir="LTR" style="font-size:10.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مواد و روش&shy;ها:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این مطالعه </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از 50 نمونه بافت شامل</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 25 نمونه بافت مبتلا به گلیوبلاستوما مولتی فرم بیماری و 25 نمونه بافت سالم اطراف تومور</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> GBM&nbsp; </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">قبل از درمان </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">به عنوان نمونه کنترل </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">جمع آوری شد. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در تمام نمونه ها، استخراج</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> RNA </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و سنتز</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""> cDNA </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">صورت گرفت. بیان ژن</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SLC1A2</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استفاده از روش </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real- time PCR </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شد.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ACTB</span></span></i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> به عنوان کنترل داخلی استفاده شد.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> آنالیزآماری</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">داده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با استفاده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ازنرم</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">افزار</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">GraphPad Prism</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نسخه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">8 </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;انجام شد</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> از منحنی</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Roc</span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> برای بررسی ارزش بیومارکری ژن</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SLC1A2</span></span></i><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">استفاده شد. </span></span><span style="font-family:"Arial","sans-serif"" lang="AR-SA"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">یافته&shy; ها</span></span></b><b><span dir="LTR" style="font-size:16.0pt"><span b="" style="font-family:" zar="">:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کاهش بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SLC1A2</span></span></i><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در نمونه های گلیوبلاستوما مولتی فرم مشاهده شد و </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیان آن با سن، جنس و اندازه تومور بیماران مقایسه شد. بیان ژن</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SLC1A2</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با سن بیماران از </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">لحاظ </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آ</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ماری بررسی و ارتباط معنادار در بین افراد بالای 50 سال و زیر 50 سال مشاهده شد (</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">05/0 </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P-value˂</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) در حالی که در جنسیت و اندازه تومور</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> این ارتباط </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">معنادار مشاهده نشد (</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">05/0 </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P-value</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">).</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">نتیجه &shy;گیری</span></span></b><b> </b><b><span dir="LTR" style="font-size:16.0pt"><span b="" style="font-family:" zar="">:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SLC1A2</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بافت توموری بیماران مبتلا به گلیوبلاستوما مولتی فرم</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کمتر از بافت سالم اطراف تومور بیان شده بود. با </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی منحنی </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ROC</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این احتمال می رود که </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">SLC1A2</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بتواند به عنوان&nbsp; مارکر زیستی مطرح شود.</span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Aim and Background</span></span></span></b><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">:</span></span></span></b> <span new="" roman="" style="font-family:" times="">glioblastoma Multiforme (GBM), astrocytoma grade IV which makes up 53% of</span> <span new="" roman="" style="font-family:" times="">all gliomas. It is the most common and deadly primary malignant brain tumor in adults. The mortality rate in glioblastoma multiforme is very high.</span> <span new="" roman="" style="font-family:" times="">The aim of this study was to determine the expression of <i>SLC1A2</i> gene in tumor tissues and margins Healthy subjects with glioblastoma multiform were designed and performed</span><span b="" dir="RTL" nazanin="" style="font-family:">.</span><span style="font-family:"Times New Roman","serif""></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Materials and Methods</span></span></span></b><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">:</span></span></span></b><b> </b><span new="" roman="" style="font-family:" times="">In this study, 50 tissue samples including 25 tissue samples with glioblastoma multiform disease and 25 healthy tissue samples around the GBM tumor before treatment were collected as control samples. RNA extraction and cDNA synthesis were performed in all samples. <i>SLC1A2</i> gene expression was investigated using Real-time PCR method. <i>ACTB</i> gene was used as internal control. Statistical analysis of data was done using GraphPad Prism version 8 software. Roc curve was used to check the biomarker value of <i>SLC1A2</i> gene.</span><span dir="RTL" style="font-family:"B Nazanin"" lang="AR-SA"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Results:</span></span></span></b><b> </b><span new="" roman="" style="font-family:" times="">Decreased <i>SLC1A2</i> gene expression was observed in glioblastoma multiforme samples and its expression was compared with the age, gender and tumor size of the patients. The expression of <i>SLC1A2</i> gene with the age of the patients was statistically investigated and a significant relationship was observed among people over 50 years old and under 50 years old (P-value˂ 0.05), while this significant relationship was not observed in gender and tumor size (P-value>0.05).</span><span dir="RTL" style="font-family:"B Nazanin"" lang="AR-SA"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:107%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></span></b><b> </b><span new="" roman="" style="font-family:" times="">The <i>SLC1A2</i> gene was less expressed in the tumor tissue of patients with glioblastoma multiforme than in the healthy tissue around the tumor. By examining the ROC curve, it is possible that the <i>SLC1A2</i> gene can be proposed as a biomarker.</span></span></span></span><br> &nbsp; ژنSLC1A2, بیومارکر,گلیوبلاستوما مولتی فرم, Iau Science. SLC1A2 Gene, Biomarker, Glioblastoma multiforme, Iau Science. 51 60 http://ncmbjpiau.ir/browse.php?a_code=A-10-1107-2&slc_lang=fa&sid=1 Nasrin Karimi نسرین کریمی 100319475328460013169 100319475328460013169 Yes Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران Hamidreza Kheiri حمیدرضا خیری h.kheiri@zums.ac.ir 100319475328460013170 100319475328460013170 No Department of Pharmaceutical Biotechnology, School of Pharmacy, Zanjan University of Medical Sciences, Zanjan, Iran Vajiheh Zarrinpour وجیهه زرین پور 100319475328460013171 100319475328460013171 No Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran Mohammadmahdi Forghanifard محمد مهدی فرقانی فرد 100319475328460013172 100319475328460013172 No Department of Biology, Damghan Branch, Islamic Azad University, Damghan, Iran