fa مقایسه اثرهای MNPs عاملدار شده با سیلیکای مزوپور و L-لیزین (NH2-Si@Fe3O4) بر تغییرهای ساختاری پروتئین لیزوزیم سفیده تخم مرغ (HEWL) فیزیولوژی Physiology مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="background:white none repeat scroll 0% 0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">جذب پروتئین برسطوح غیرآلی، منجر</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به تغییرات ساختاری و عملکردی می&shy;گردد که وابسته به طبیعت پروتئین جذب&shy;شده و خصوصیات فیزیکوشیمیایی&shy;سطوح غیرآلی&shy;است</span></span>.<span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نانوذرات&shy;مغناطیسی (</span></span><span dir="LTR" style="font-size:10.0pt">MNPs</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) با پوشش&shy;های زیست&shy;سازگار تنها مواد نانوساختار مورد تأیید </span></span><span dir="LTR" style="font-size:10.0pt">FDA</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> هستند که با کاربردهای</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">متنوع در زمینه های مختلف علوم پزشکی و خصوصیات ویژه به ماده&shy;ای با پتانسیل بالا تبدیل شده&shy;اند. بررسی ایمنی زیستی </span></span><span dir="LTR" style="font-size:10.0pt">MNPs</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، نیاز به &shy;دانش دقیق در مورد برهمکنش </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt">MNPs</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با مولکول&shy;های زیستی به ویژه پروتئین&shy;ها می&shy;باشد</span></span>.<span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> مطالعه حاضر، مقایسه اثرات</span></span> <span dir="LTR" style="font-size:10.0pt">MNPs</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">عاملدار شده با سیلیکای مزوپور و </span></span><span dir="LTR" style="font-size:10.0pt">L</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">-لیزین </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">(</span></span><span dir="LTR" style="font-size:10.0pt">NH₂</span>-<span dir="LTR" style="font-size:10.0pt">Si@Fe₃O₄</span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر تغییرات ساختاری پروتئین لیزوزیم سفیده تخم&shy;مرغ </span></span>(<span dir="LTR" style="font-size:10.0pt">HEWL</span>)<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> را ارائه می&shy;دهد.</span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white none repeat scroll 0% 0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">مواد و روش&shy;ها</span></span></b><b><span dir="LTR" style="font-size:14.0pt">:</span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">خصوصیات</span></span>&shy;<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ساختاری وشاخص&shy;های فیزیکوشیمیایی</span></span>MNPs<span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> عاملدار شده توسط آنالیزهای</span></span><span dir="LTR" style="font-size:10.0pt">FTIR</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">XRD</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:10.0pt">TEM</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" style="font-size:10.0pt">FESEM</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span dir="LTR" style="font-size:10.0pt">VSM</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt">DLS</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برهم&shy;کنش پروتئین-نانوذرات با</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">استفاده از تکنیک&shy;های فلورسانس ذاتی، تست </span></span><span dir="LTR" style="font-size:10.0pt">ANS</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، تیوفلاوین&shy;تی (</span></span><span dir="LTR" style="font-size:10.0pt">ThT</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)، </span></span><span dir="LTR" style="font-size:10.0pt">ATR-FTIR</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تجمع </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">حرارتی</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> پروتئین</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">انجام شد.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تغییر ساختار</span></span><span dir="LTR" style="font-size:10.0pt">HEWL</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> برتعامل با</span></span> <span dir="LTR" style="font-size:10.0pt">MNPs</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مطالعه گردید</span></span>. <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white none repeat scroll 0% 0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">یافته &shy;ها</span></span></b><b><span dir="LTR" style="font-size:14.0pt">:</span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی تعامل </span></span><span dir="LTR" style="font-size:10.0pt">HEWL</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و</span></span> <span dir="LTR" style="font-size:10.0pt">MNPs</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با کمک فلورسانس ذاتی، نشان داد اتصال پروتئین&shy;ها با </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt">MNPs</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تحت تأثیر نوع لیگاند متصل به</span></span> <span dir="LTR" style="font-size:10.0pt">MNPs</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">است. غربالگری تجمع حرارتی </span></span><span dir="LTR" style="font-size:10.0pt">HEWL</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> درحضور</span></span><span dir="LTR" style="font-size:10.0pt">MNPs</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، نقش&shy;مؤثر </span></span><span dir="LTR" style="font-size:10.0pt">NH₂-Si@Fe₃O₄</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">را در مهار تجمع حرارتی </span></span><span dir="LTR" style="font-size:10.0pt">HEWL</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بیان نمود</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white none repeat scroll 0% 0%"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">نتیجه گیری</span></span></b><b><span dir="LTR" style="font-size:14.0pt">:</span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقایسه&shy; روی عملکرد نانوذرات با گروه عاملی و بدون گروه عاملی، نشان داد نانوذره عاملدار شده عملکرد بهتری داشته است، لذا مهم است جنبه&shy;های محیطی، سلامتی و ایمنی در مراحل اولیه استفاده از نانوذرات درنظر گرفته شود. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این گزارش یک تصویر یکپارچه از تعامل پروتئین و </span></span><span dir="LTR" style="font-size:10.0pt">MNPs</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و تغییر ساختار پروتئین پس از اتصال به</span></span> <span dir="LTR" style="font-size:10.0pt">MNPs</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">را نشان داد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <br> &nbsp; <span style="font-size:11pt"><span style="background:white none repeat scroll 0% 0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b>Aim and Background</b>: Adsorption of protein on inorganic surfaces leads to structural and functional changes that depend on the nature of the adsorbed protein and the physicochemical properties of inorganic surfaces. Magnetic nanoparticles (MNPs) with biocompatible coatings are the only FDA-approved nanostructured materials with various applications in medical sciences and unique properties have become a high potential material. Study of the bioimmunity of MNPs requires detailed knowledge of the interaction of MNPs with biological molecules, especially proteins. The present study, Comparison of the effects of functionalized MNPs by mesoporous silica and L-lysine (NH₂-Si@Fe₃O₄) on structural changes of egg white lysozyme protein (HEWL<b>)</b><b>.</b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white none repeat scroll 0% 0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b>Material and Methods:</b> Structural properties and physicochemical characteristics of functionalized MNPs by FTIR, XRD, TEM, FESEM, VSM and DLS analyzes and Protein-nanoparticle interaction was performed using intrinsic fluorescence techniques, ANS test, thioflavin T (ThT) and ATR-FTIR and protein thermal aggregation. Structure change of HEWL on interaction with MNPs was studied.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white none repeat scroll 0% 0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b>Conclusion:</b> Investigation of the interaction of HEWL and MNPs with the help of intrinsic fluorescence showed that the binding of proteins to MNPs is influenced by the type of ligand attached to MNPs. Screening thermal aggregation of HEWL in the presence of MNPs revealed the effective role of NH2-Si@Fe3O4 in inhibiting thermal aggregation of HEWL.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="background:white none repeat scroll 0% 0%"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span style="color:black"><b>Results:</b> Comparison of nanoparticle performance with functional group and without functional group showed that functionalized nanoparticles had better performance; therefore, it is important to consider the environmental, health and safety aspects in the early stages of nanoparticle use. This report provides an integrated picture of protein-MNPs interaction and the change in protein structure after binding to MNPs.</span></span></span></span></span><br> <br> <br> <br> <br> &nbsp; نانوذرات مغناطیسی, سیلیکای مزوپور, L-لیزین, پروتئین لیزوزیم, .Iau Science Magnetic nanoparticles, Mesoporous silica, L-lysine, Lysozyme protein, Iau Science. 99 114 http://ncmbjpiau.ir/browse.php?a_code=A-10-1724-3&slc_lang=fa&sid=1 Forough Zakernezhad فروغ ذاکرنژاد 100319475328460013583 100319475328460013583 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, I.R. of Iran گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران Behnam Rasekh بهنام راسخ 100319475328460013584 100319475328460013584 No Environment & Biotechnology Division, Research Institute of Petroleum Industry (RIPI), P.O. Box: 14665-137, Tehran, Iran گروه پژوهش میکروبیولوژی و بیوتکنولوژی، پژوهشگاه صنعت نفت، تهران، ایران Parvaneh Maghami پروانه مقامی maghami.p@ut.ac.ir 100319475328460013585 100319475328460013585 No Department of Biophysics, Science and Research Branch, Islamic Azad University, Tehran, I.R. of Iran گروه بیوفیزیک، دانشگاه آزاد اسلامی واحد علوم و تحقیقات، تهران، ایران Fatemeh Yazdian فاطمه‌ یزدیان 100319475328460013586 100319475328460013586 Yes Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran گروه مهندسی علوم‌ زیستی، دانشکده علوم و فنون نوین، دانشگاه تهران، تهران، ایران