fa رابطه لپتین سرم و مقاومت به انسولین در زنان چاق ایرانی فیزیولوژی Physiology مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:105%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">سابقه و هدف</span></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:105%"><span calibri="" style="font-family:"><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">فرایند چاقی پیچیده است وعوامل متعدد از جمله دو هورمون کلیدی&nbsp; لپتین و انسولین&nbsp; در آن دخیل هستند. نقس هومئوستاتیک انسولین در ثبات انرژی&nbsp; با مقاومت به انسولین کاهش می یابد. احتمال دارد&nbsp; که لپتین و انسولین بر یکدیگر نقش تنظیمی داشته باشند. لذا، مطالعه حاضر به بررسی رابطه بین شاخص توده بدن، لپتین و انسولین&nbsp; و مقاومت به انسولین پرداخته است.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:105%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">مواد و روش ها</span></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:105%"><span calibri="" style="font-family:"><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">در این مطالعه مقطعی، 140 زن در 2 گروه مساوی با شاخص توده بدنی نرمال (9/24-5/18) یا چاق (</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">BMI &ge; 30</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">) مورد بررسی قرار گرفتند. پس از 10 تا 12 ساعت ناشتایی، 10 سی سی خون وریدی از افراد گرفته شد، قند خون با استفاده از گلوکز اکسیداز، انسولین و لپتین به روش الایزا اندازه گیری شد و شاخص </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">HOMA-IR</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:"> محاسبه شد و داده ها به صورت&nbsp; میانگین </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">&plusmn;</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:"> انحراف معیار بیان گردید </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">.</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">&nbsp;&nbsp; 05 /0</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">></span></span></span> <span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">P</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:"> معنی دار در نظر گرفته شد.</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:105%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">یافته‌ها:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:"> بین گروه‌ها از نظر سطح لپتین تفاوت معنی‌داری وجود داشت و بین شاخص توده بدن و سطح لپتین پلاسمایی آزمودنی‌های مورد مطالعه همبستگی&nbsp; مثبت شدید وجود داشت(7/0 </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">r=</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:"> و.01/0</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">P</span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:Aharoni"><</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">. همچنین بین لپتین سرم و انسولین ناشتا همبستگی مثبت متوسط وجود داشت (05/0</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">r</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">= و 01/0< </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">P</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">)</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">. از نظر </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">HOMA-IR</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:"> تفاوت معنی‌داری بین گروه‌ها مشاهده شد که در افراد چاق بیشتر از گروه عادی بود<span style="color:red">).</span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:105%"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:">نتیجه گیری</span></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:105%"><span calibri="" style="font-family:"><span dir="LTR" style="font-size:12.0pt"><span style="line-height:105%">BMI</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:105%"><span b="" nazanin="" style="font-family:"> عامل اصلی تنظیم سطح لپتین است. احتمالاً پیوستگی سطح انسولین و لپتین به دلیل نقش تعیین کننده شاخص توده بدن بر هر دوی آنها است.</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:105%"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">Aim and </span></span></span></b><b><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">Background </span></span></span></b><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"Times New Roman",serif"></span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:105%"><span calibri="" style="font-family:"><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">The process of obesity is complex and many factors, including two key hormones, leptin and insulin are involved. The homeostatic role of insulin in energy stability is reduced by insulin resistance. It is possible that leptin and insulin have a regulatory role on each other, therefore, the present study investigated the relationship between BMI, leptin , insulin and insulin resistance.</span></span></span><span style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:105%"><span calibri="" style="font-family:"><b><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">Materials and methods</span></span></span></b><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"Times New Roman",serif"></span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:105%"><span calibri="" style="font-family:"><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">In this cross-sectional study, 140 women were examined in 2 equal groups with normal (18.5-24.9) or obese (&ge; 30) BMI. After fasting for 10-12 hours, 10 cc of venous blood was taken from the subjects, Blood sugar was measured by glucose oxidase, insulin and leptin by ELISA method, and HOMA-IR index was calculated</span></span></span><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">. </span></span></span><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">The data were expressed as mean &plusmn; standard deviation. P < 0.05 was considered </span></span></span><em><span style="font-size:12.0pt"><span style="background:white"><span style="line-height:105%"><span style="color:black">statistically </span></span></span></span></em><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">significant.</span></span></span><span style="font-size:12.0pt"><span style="line-height:105%"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:105%"><span calibri="" style="font-family:"><b><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">Results</span></span></span></b><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">: There was a significant difference between the groups in terms of leptin level, and there was a significant correlation between BMI and plasma leptin level of the studied subjects, r=0.07 and P<0.01. Also, there was a significant relationship between serum leptin and fasting insulin (r = 0.05 and P < 0.01. In terms of the HOMA-IR, a significant difference between the groups was observed which was higher in obese than in normal.</span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:105%"><span calibri="" style="font-family:"><b><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:105%"><span calibri="" style="font-family:"><span lang="EN" style="font-size:12.0pt"><span style="line-height:105%"><span new="" roman="" style="font-family:" times="">BMI is the main factor in regulating leptin levels. Probably the continuity of insulin and leptin levels is due to the determining role of BMI on both of them.</span></span></span></span></span></span><br> <br> &nbsp; لپتین , انسولین , چاقی , زنان,Iau Science Isulin, Leptin, Obesity, Women,Iau Science 23 32 http://ncmbjpiau.ir/browse.php?a_code=A-10-1787-2&slc_lang=fa&sid=1 Mina Jaberi مینا جابری 100319475328460014258 100319475328460014258 Yes Department of Biolgy, Islamshahr Branch , Islamic Azad University, Islamshahr, Iran گروه زیست شناسی،دانشکده علوم پایه،دانشگاه آزاد اسلامی واحد اسلامشهر، اسلامشهر، ایران Elham Asadi الهام اسدی elham.asadi.2@gmail.com 100319475328460014259 100319475328460014259 No Department of Biolgy, Islamshahr Branch , Islamic Azad University, Islamshahr, Iran گروه زیست شناسی،دانشکده علوم پایه،دانشگاه آزاد اسلامی واحد اسلامشهر، اسلامشهر، ایران