fa بررسی ترکیبات آنتی اکسیدانی و ضد باکتریایی عصاره برگ، گل و ریشه گیاه آقطی (Sambucus ebulus) و اثر سمیت آن‌ها بر سلول‌های سرطانی ریه سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">چکیده</span></span></span></b><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سابقه و هدف:</span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> امروزه</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سرطان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">یکی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">از</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">علل</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">اصلی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مرگ</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">و</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">میر</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">در</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">جهان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">می&shy;باشد.</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">ترکیبات</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">گیاهان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">دارویی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">القاکننده</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">آپوپتوز،</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">از روش&shy;های</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">درمان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سرطان</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">محسوب</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">می&shy;گردد. همچنین</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">با افزایش روز افزون مقاومت باکتری&shy;ها به انواع آنتی بیوتیک&shy;&shy;ها، استفاده از گیاهان دارویی در درمان عفونت&shy;های باکتریایی رو به افزایش است.</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> هدف این پژوهش بررسی ترکیبات آنتی اکسیدانی و ضد باکتریایی عصاره&shy;های برگ، گل و ریشه گیاه آقطی و اثر مهاری آن‌ها بر</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">روی</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سلول&shy;های سرطانی ریه می&shy;باشد.<b> </b></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مواد و روش&shy;ها: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بخش&shy;های مختلف گیاه آقطی از شهر عباس آباد جمع آوری و عصاره&shy;گیری به روش خیساندن انجام شد. جهت تعیین سمیت سلولی عصاره&shy;ها از روش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">MTT</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> و </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">فعالیت ضد میکروبی عصاره&shy;ها از روش&shy;ها انتشار دیسک استفاده شد. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">همچنین میزان فنل، فلاونوئید و آنتوسیانین کل عصاره&shy;ها نیز تعیین شد. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">یافته ها:</span></span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتایج نشان داد بیشترین محتوای فنل کل در عصاره&shy; برگ بدست آمد. تاثیر عصاره&shy;ها بر روی مهار رشد سلول&shy;ها وابسته به غلظت و زمان بود. بیشترین درصد مهار رشد سلول&shy;ها در غلظت 4000 میکروگرم بر میلی&shy;لیتر در عصاره&shy;های برگ و گل به ترتیب 47/97 و 87/97 درصد در مدت زمان 72 ساعت بدست آمد.</span></span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">همچنین، بیشترین اثر مهاری عصاره&shy;های برگ و گل بر روی باکتری گرم مثبت <i>استافیلوکوس اورئوس</i> در غلظت 1000میلی&shy;گرم بر میلی&shy;لیتر بدست آمد. </span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتیجه‌گیری:</span></span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بنابراین نتایج این مطالعه پیشنهاد می&shy;کند که عصاره&shy; گیاه آقطی به ویژه عصاره برگ و گل می&shy;تواند به عنوان یک منبع قابل دسترس از ترکیبات آنتی&shy;اکسیدان باشد و به علت داشتن خاصیت ضد سرطانی بالا می&shy;تواند به عنوان یک گیاه دارویی علیه سرطان ریه مورد پژوهش&shy;های وسیع&shy;تری قرار گیرد. </span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Abstract</span></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Aim and Background</span></span></b><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">:</span></span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Cancer is considered one of the leading causes of mortality worldwide. Components derived from natural plants that induce apoptosis are used for cancer treatment.</span></span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> Also,</span></span></span> <span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">with the increasing resistance of bacteria to antibiotics and their side effects, the use of medicinal plants in the treatment of bacterial infections is increasing.</span></span></span> <span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Therefore, this study aims to evaluate the antioxidant and antibacterial properties of the leaves, flowers, and roots extract of <i>Sambucus ebulus</i> and their inhibitory effect on lung cancer cells. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt"><span calibri="" style="font-family:"><b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></span></b> <span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#202124">Different parts of </span></span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">the </span></span></span><i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">S. ebulus</span></span></i><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> were collected </span></span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">from </span></span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#202124">Abbas Abad city and extracted by the maceration method. To determine the cytotoxicity of the extracts, the MTT method was used, and </span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">the antimicrobial activity of the extracts</span></span></span><span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black"> was determined by disk diffusion methods.</span></span></span> <span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:black">Also, total phenol, flavonoid, and anthocyanin contents were measured.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:45.8pt 91.6pt 137.4pt 183.2pt 229.0pt 274.8pt 320.6pt 366.4pt 412.2pt 458.0pt 503.8pt 549.6pt 595.4pt 641.2pt 687.0pt 732.8pt"><span calibri="" style="font-family:">&nbsp;<b><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b> <span lang="EN" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#202124">The results showed that the highest content of total phenol was obtained from the leaf extract. The effect of the extracts on cell growth inhibition was concentration and time-dependent; the highest percentage of cell growth inhibition at the concentration of 4000 &mu;g/mL in leaf and flower extracts was 97.47% and 97.87%, respectively, in the duration of 72 hours. Also, the maximum inhibitory effect of leaf and flower extracts on Gram-positive bacteria, <i>Staphylococcus aureus,</i> was obtained at a concentration of 1000 mg/mL.</span></span></span><span style="font-size:10.0pt"><span new="" roman="" style="font-family:" times=""><span style="color:#202124"></span></span></span></span></span></span></span> <pre style="text-align:justify"> <span style="font-size:10pt"><span style="font-family:Consolas"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> The results of this study suggest that especially the leaf and flower extract of <i>S</i></span><i><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span></i><i><span new="" roman="" style="font-family:" times=""> ebulus</span></i><span new="" roman="" style="font-family:" times=""> plant can be an accessible source of antioxidant compounds and can be used as a medicinal plant against lung cancer, subject to extensive research due to its high anti-cancer properties.</span><span style="font-family:"Times New Roman","serif""></span></span></span></pre> واژگان کلیدی: آقطی, آنتی‌اکسیدان, سرطان ریه, فعالیت ضدباکتریایی. Keywords: Sambucus ebulus, Antioxidant compounds, Lung cancer, Antibacterial properties. 27 40 http://ncmbjpiau.ir/browse.php?a_code=A-10-1993-2&slc_lang=fa&sid=1 Amin Tavakoli Yaraki امین توکلی یرکی Azarzamani24@gmail.com 100319475328460015676 100319475328460015676 No . Master's student, Department of Microbiology, To.C., Islamic Azad University,Tonekabon, Iran. 1. دانشجوی کارشناسی ارشد، گروه میکروبیولوژی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران. Simin Arian سیمین آرین Simin.aryan@yahoo.com 100319475328460015677 100319475328460015677 Yes Assistant Professor, Department of Microbiology, To.C., Islamic Azad University,Tonekabon, Iran. 2. استادیار، گروه میکروبیولوژی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران. Mahdieh Houshani مهدیه هوشنی mhoshani@yahoo.com 100319475328460015678 100319475328460015678 No استادیار، گروه فیزیولوژی گیاهی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران