Volume 7, Issue 27 (9-2017)                   NCMBJ 2017, 7(27): 69-76 | Back to browse issues page

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shahhosseiny A, mahasti shotorbani P, akhundzadeye basti A. Rapid molecular detection of Listeria monocytogenes in Iran industrial sauce. NCMBJ 2017; 7 (27) :69-76
URL: http://ncmbjpiau.ir/article-1-1003-en.html
Department of food industry scince and research branch , Islamic azad universit , Tehran, iran , ati.shahhosseiny@yahoo.com
Abstract:   (4540 Views)
Aim and Background: Listeria monocytogenes is known as a food-borne pathogen responsible for causing disease in humans. This bacterium that cause meningitis, abortion and food poisoning in humans. Industrial sauce as a matter of day-to-day offer food consumption among the general population have found. This food, suitable for food poisoning. Therefore contamination with Listeria monocytogenes is a threat to health, especially those with suppressed immune systems. The aim of this study is molecular detection of listeria monocytogenes in industrial sauce in Iran. Materials and Methods: 25 Industrial sauces were collected from Iran markets. DNA samples extracted by standard phenol-chloroform method . PCR test optimized and detection of limits (LOD) and specificity evaluated. All of the samples tested with optimized PCR test. Results: PCR test Optimized and 226 bp amplicon was observed in 2% agarose gel electrophoresis. LOD test was obtained 10 genome/reaction and in specificity test, amplicon was not observed with none of microorganism DNA was tested. After PCR testing on 25 sauce samples,4 were positive for Listeria monocytogenes. Conclusion: PCR technique using specific primers very carefully and faster than traditional methods is for the identification of Listeria monocytogenes. The results show that the PCR technique is a good way to monitor microorganisms in food products such as sauces.
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Type of Study: Research Article | Subject: Genetics
Received: 2017/10/24 | Accepted: 2017/10/24 | Published: 2017/10/24

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