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:: Volume 7, Issue 28 (10-2017) ::
NCMBJ 2017, 7(28): 49-60 Back to browse issues page
An evaluation of the transmembrane ammonium sulfate gradients method in lipid system to improve trapping capacity of amphipathic weak base drugs
Bibi Fatemeh Haghiralsadat , Ghasem Amoabediny , Samira Naderinezhad , Mohammad-hasan Sheikhha , Zahra Malaei-Balasi , Azim Akbarzade , Behrouz Zandieh Doulabi
2. Department of Nano Biotechnology, Research center for new technologies in life science engineering, University of Tehran, Tehran, Iran. , amoabedini@ut.ac.ir
Abstract:   (1272 Views)
Aim and Background:
Liposomal vesicles provide the possibility of loading lipophilic drugs into phospholipids bilayers and water-soluble (hydrophilic) aqueous phase. The present study was aimed on the evaluation of the properties of liposomal doxorubicin loaded into liposome by both active and passive procedures in terms of drug loading and release.
Material and methods:
Dipalmitoyl glycerol phospholipid glycerol, cholesterol and DSPE-mPEG 2000 was used for synthesis of nanoliposome. Then, doxorubicin loading was performed by passive (thin film hydration) and active (according to pH gradient) methods. The average diameter of nanoparticls was measured with Zeta Sizer. And the amount of drug loading and release was performed using dialysis.
The average size of nanoparticles were 138.6 ± 4.9 nm  and 105.9 ± 3.8 nm for thin film hydration method, and pH gradient method, respectively. And drug loading efficacy of doxorubicin-containing nanoliposomes was 89±4.35 for pH gradient method and 15.65±8.65 for thin film method. The accumulated amount of drug release during 48 hours in PBS at pH=7.4, was determined 78% and 24% for hydration thin film method pH gradient method, respectively.
This study shows that nanoliposomal doxorubicin prepared by active method, was more effective than that the passive method. Our prepared nanoformulation was also sensitive to pH of medium.
Keywords: pH gradient, Doxorubicin, Liposome, pH sensitive.
Full-Text [PDF 545 kb]   (396 Downloads)    
Type of Study: Research Article | Subject: Pharmacology
Received: 2017/12/30 | Accepted: 2017/12/30 | Published: 2017/12/30
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Haghiralsadat B F, Amoabediny G, Naderinezhad S, sheikhha M, Malaei-Balasi Z, Akbarzade A et al . An evaluation of the transmembrane ammonium sulfate gradients method in lipid system to improve trapping capacity of amphipathic weak base drugs . NCMBJ. 2017; 7 (28) :49-60
URL: http://ncmbjpiau.ir/article-1-1032-en.html

Volume 7, Issue 28 (10-2017) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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