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:: Volume 8, Issue 29 (1-2018) ::
NCMBJ 2018, 8(29): 31-38 Back to browse issues page
Assessment of virulence factors(elastase and phospholipase) and antibiotic susceptibility in Pseudomonas aeruginosa isolated from clinical samples
Mahya Rahbar zare , Fatemeh Noorbakhsh , Sahar Honarmand jahromi
,Department of Microbiology, Biological Science College, Varamin-pishva branch,Islamic Azad University,Varamin-Pishva,Iran , niloofar_noorbakhsh@yahoo.com
Abstract:   (718 Views)
Aim and Background:
 Pseudomonas aeruginosa is one of the most important pathogen cause infection in hospital in world. Different virulence gene exsist in this bacteria, which interfere in pathogenesis and infection.
 The aim of this study was evaluation of antibiotic susceptibility pattern and identify virulence genes plcH, lasB, plcN of P. aeruginosa isolated from clinical samples.
 
Material and methods:
 In this study 100 Pseudomonas aeruginosa isolated of human cases of burn in 1395.
Antibiotic susceptibility test was performed by disk diffusion method. Genomic DNA were extracted from isolated strains and Multiplex- PCR performed to determine the preavalence  of each of the tested genes.
Result: The scope age of  patients were 20-90 years, and 46 patient were male and 52 were female.The prevalence of plcN and plcH  observed  98 percent and lasB 100 percent.  The highest resistance to antibiotics were observed in  ampicillin and cefoxitin with the frequency of  93% and 96% respectively.
Conclusion:The  high prevalence of plcH, lasB, plcN gene in Pseudomonas aeruginosa isolated of skin case of burn revealed that this genes are an important virulence factors in burn infections.
Keywords: Pseudomonas aeruginosa, plcH, lasB, plcN
Full-Text [PDF 457 kb]   (167 Downloads)    
Type of Study: Research Article | Subject: Microbiology
Received: 2018/02/27 | Accepted: 2018/02/27 | Published: 2018/02/27
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rahbar zare M, Noorbakhsh F, Honarmand jahromi S. Assessment of virulence factors(elastase and phospholipase) and antibiotic susceptibility in Pseudomonas aeruginosa isolated from clinical samples. NCMBJ. 2018; 8 (29) :31-38
URL: http://ncmbjpiau.ir/article-1-1055-en.html


Volume 8, Issue 29 (1-2018) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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