Volume 8, Issue 29 (1-2018)
NCMBJ 2018, 8(29): 69-78 |
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Kaviani B, Sazgar H, Zia Jahromi N, Mohamadi Farsani F. Investigation of drug resistance against treatment with Enzalutamide medicine in individuals diagnosed with prostate cancer and studying the effect of rs137852574 single-nucleotide polymorphism in drug resistance in the human population of Isfahan province. NCMBJ 2018; 8 (29) :69-78
URL: http://ncmbjpiau.ir/article-1-1059-en.html
URL: http://ncmbjpiau.ir/article-1-1059-en.html
Department of Biology, Science Faculty, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran , hoseinsazgar@yahoo.com
Abstract: (6166 Views)
Aim and Background:
The purpose of this study is to investigate role of rs137852574 single-nucleotide polymorphism in the androgen receptor coding gene on drug resistance of patients with prostate cancer against Enzalutamide.
Material and methods:
In this study, the ARMS-PCR analysis was conducted on androgen receptor coding gene in 50 patients with prostate cancer with drug resistance and 50 patients with prostate cancer without drug resistance. Blood samples were collected from patients admitted to Omid and al-Zahra hospitals of Isfahan province.
Results:
The allele frequencies of T and G alleles in rs137852574 were 0.72 and 0.28 for drug resistant and 0.88 and 0.12 for non-drug resistance groups. In addition, Evaluation of heterozygosity showed that the marker was in Hardy-Weinberg equilibrium.
Conclusion:
The results indicate that there is a meaningful relationship between drug resistance and rs137852574 single-nucleotide polymorphism existence (P-Value = 0.039). Moreover, results gained from investigating the drug resistance mechanism using the docking technique showed that in individuals with rs137852574 single-nucleotide polymorphism, the drug is not accurately bonded to the active pocket of androgen receptor, and consequently this drug’s binding energy to the receptor is reduced.
The purpose of this study is to investigate role of rs137852574 single-nucleotide polymorphism in the androgen receptor coding gene on drug resistance of patients with prostate cancer against Enzalutamide.
Material and methods:
In this study, the ARMS-PCR analysis was conducted on androgen receptor coding gene in 50 patients with prostate cancer with drug resistance and 50 patients with prostate cancer without drug resistance. Blood samples were collected from patients admitted to Omid and al-Zahra hospitals of Isfahan province.
Results:
The allele frequencies of T and G alleles in rs137852574 were 0.72 and 0.28 for drug resistant and 0.88 and 0.12 for non-drug resistance groups. In addition, Evaluation of heterozygosity showed that the marker was in Hardy-Weinberg equilibrium.
Conclusion:
The results indicate that there is a meaningful relationship between drug resistance and rs137852574 single-nucleotide polymorphism existence (P-Value = 0.039). Moreover, results gained from investigating the drug resistance mechanism using the docking technique showed that in individuals with rs137852574 single-nucleotide polymorphism, the drug is not accurately bonded to the active pocket of androgen receptor, and consequently this drug’s binding energy to the receptor is reduced.
Type of Study: Research Article |
Subject:
Genetics
Received: 2018/02/27 | Accepted: 2018/02/27 | Published: 2018/02/27
Received: 2018/02/27 | Accepted: 2018/02/27 | Published: 2018/02/27
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