Volume 8, Issue 30 (4-2018)
NCMBJ 2018, 8(30): 17-24 |
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Naderinezhad S, Haghiralsadat F, Amoabediny , G, Rajaei Najafabadi S, Akbarzade A. Synthesis of sustained-release niosomal Doxorubicin and investigation of effective drug dose in nano-formula against bone marrow cancer. NCMBJ 2018; 8 (30) :17-24
URL: http://ncmbjpiau.ir/article-1-1080-en.html
URL: http://ncmbjpiau.ir/article-1-1080-en.html
Samira Naderinezhad 
, Fateme Haghiralsadat , , Ghasem Amoabediny , Saeideh Rajaei Najafabadi , Azim Akbarzade
, Fateme Haghiralsadat , , Ghasem Amoabediny , Saeideh Rajaei Najafabadi , Azim Akbarzade
(1) Department of Biotechnology and Pharmaceutical Engineering, School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran , amoabedini@ut.ac.ir
Abstract: (6697 Views)
Aim and Background: Because of high half- life of niosomes in blood plasma makes the possibility of delivery of chemotherapy agent to tumor tissue and improves the therapeutic index of chemotherapy drug. In this study, the nano-nisomes contained doxorubicin was synthesized. The effective dose of drug was determined and anti-cancer effect of resulting nanoparticle was evaluated.
Material & Methods: Niosome containing cholesterol, span 60 with the 85:15 molar ratios were prepared by thin film hydration method. In the hydration step, various concentration of doxorubicin diluted with distillated water were used to determine the optimized drug concentration. The cellular cytotoxicity was finally examined using MTT assay.
Results: The results imply that optimized drug dose is 0.5mg/ml. The entrapment efficiency; size diameter and polydispersity index of optimal formulation are 81.69, 102.9 and 0.128, respectively. The amount of drug release is 35% during 144 hours. The prepared system reduces the side effects of doxorubicin and be effective against cancer cells.
Discussion: This study showed that the optimal dose of drug plays an important role in improving the percent of drug loading that is economically optimal. Also it leads to expose the patient's body with the lower doses of medication and the most therapeutic effect. Reduce the dose of medication also causes less damage to healthy cells. Sustained-release property of system is the main reason for the increased toxicity of chemotherapy drug.
Conclusions: Prepared niosomal system is slow release with size diameter less than 150 nm and high drug entrapment efficacy that can be used to overcome the side effect of free doxorubicin. The resulting system is effective against bone marrow cancerous cells.
Material & Methods: Niosome containing cholesterol, span 60 with the 85:15 molar ratios were prepared by thin film hydration method. In the hydration step, various concentration of doxorubicin diluted with distillated water were used to determine the optimized drug concentration. The cellular cytotoxicity was finally examined using MTT assay.
Results: The results imply that optimized drug dose is 0.5mg/ml. The entrapment efficiency; size diameter and polydispersity index of optimal formulation are 81.69, 102.9 and 0.128, respectively. The amount of drug release is 35% during 144 hours. The prepared system reduces the side effects of doxorubicin and be effective against cancer cells.
Discussion: This study showed that the optimal dose of drug plays an important role in improving the percent of drug loading that is economically optimal. Also it leads to expose the patient's body with the lower doses of medication and the most therapeutic effect. Reduce the dose of medication also causes less damage to healthy cells. Sustained-release property of system is the main reason for the increased toxicity of chemotherapy drug.
Conclusions: Prepared niosomal system is slow release with size diameter less than 150 nm and high drug entrapment efficacy that can be used to overcome the side effect of free doxorubicin. The resulting system is effective against bone marrow cancerous cells.
Type of Study: Research Article |
Subject:
Biochemistry
Received: 2018/04/23 | Accepted: 2018/04/23 | Published: 2018/04/23
Received: 2018/04/23 | Accepted: 2018/04/23 | Published: 2018/04/23
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