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:: Volume 8, Issue 30 (4-2018) ::
NCMBJ 2018, 8(30): 71-78 Back to browse issues page
Isolation and molecular identification of resistant bacteria Pb from soils oil contaminated in area C-branch Masjed Soleiman city in Khuzestan
Maedeh Sajadian , Monir Doudi , Abbas Shirmardi
Department of Microbiology, Falavarjan Branch, Islamic Azad University, Isfahan, Iran. , Monirdoudi@yahoo.com
Abstract:   (1025 Views)
Aim and Background:
 
Due to the high prevalence of diseases caused by the presence of lead in soil contaminated with petroleum industrial centers, then transfer the metal to plants and animals and eventually humans and high risks for human health and other living things. It seems that identify and isolate microorganisms with high strength steel and the biological removal can be an important step in the evolution of contaminated soil cleanup systems considered industrial centers.
 
Material and methods:
 
The was sampling  of three areas of oil contaminated  soils  C-branch     Masjed Soleiman. To isolate resistant bacteria lead ΙΙ PHG agar dilution method and the culture medium containing concentrations of 0.5, 1, 2, 2.5, 3, 3.5  and 4 mMol per liter of lead were used. To identify of resistant bacteria lead , first was used of biochemical tests and then  molecular methods PCR- colony.
 
 
Results:
 
In this study,  5 resistant bacteria lead from oil contaminated soils were isolated and identified that among of them   Proteus mirabilis strain AOUC-001 bacteria  and showed maximum resistance to lead (MIC=4mM).
 
Discussion and Conclusion:
 
According to the bacteria examined in this study could lead bioabsorption in -vitro and growth rather appropriate in the presence of the metal showed, so it seems a good candida for bioremedation of lead from oil contaminated soils in the future. Provided that the contaminated soils to isolate the bacteria to be filtered out treatment.
Keywords: Heavy metal resistant bacteria, Pb, Oil contaminated soils, Molecular identification, Minimum inhibitory concentration (MIC)
Full-Text [PDF 404 kb]   (249 Downloads)    
Type of Study: Research Article | Subject: Microbiology
Received: 2018/04/23 | Accepted: 2018/04/23 | Published: 2018/04/23
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Sajadian M, Doudi M, Shirmardi A. Isolation and molecular identification of resistant bacteria Pb from soils oil contaminated in area C-branch Masjed Soleiman city in Khuzestan . NCMBJ. 2018; 8 (30) :71-78
URL: http://ncmbjpiau.ir/article-1-1083-en.html


Volume 8, Issue 30 (4-2018) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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