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:: Volume 8, Issue 30 (4-2018) ::
NCMBJ 2018, 8(30): 85-94 Back to browse issues page
Detection of TEM beta-lactamase Gene in Escherichia Coli Isolated from Urinary Tract Infection of referred to the clinic boarding Robat Karim to the molecular method
Parvin Mousavi , Saeed Zaker Bostan Abad , Reza Mirnejad
Department of Biology, , Islamic Azad University, Parand Branch, Tehran, Iran , saeedzaker20@yahoo.com
Abstract:   (1168 Views)
Aim and Background: Urinary tract infections is one of the most common infectious diseases whichmany factors are involved, but bacteria such as E.coli is the most important agent of urinary tract infections. Antibiotic resistance as a major problem in the treatment and control of these infections is considered. The aim of this study was to determine the genes that cause resistance to betalactam family of antibiotics on E.coli isolated from urinary tract infections in Robat karim city.
Material & Methods:  In the present Cross-sectional study which was conducted over a period of
6 months, 123 samples of E.coli were collected from Robat karim  hospitals for molecular
analysis of TEM genes, causing antibiotic resistance by (PCR) method.Data were
analyzed using SPSS statistical test.
Results: PCR showed that the gene frequency of TEM (80%) and the highest and lowest prevalent of genes were related toTEM in E.coli isolated from urinary tract infections respectively.
Conclusion: According to the high prevalence of resistance to beta-lactam antibiotics, the current study showed that the noted geneTEM play an important role in facilitating the spread of antimicrobial resistance in this region
Keywords: TEM, E, coli, ESBL
Full-Text [PDF 548 kb]   (227 Downloads)    
Type of Study: Research Article | Subject: Genetics
Received: 2018/04/23 | Accepted: 2018/04/23 | Published: 2018/04/23
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Mousavi P, Zaker Bostan Abad S, Mirnejad R. Detection of TEM beta-lactamase Gene in Escherichia Coli Isolated from Urinary Tract Infection of referred to the clinic boarding Robat Karim to the molecular method. NCMBJ. 2018; 8 (30) :85-94
URL: http://ncmbjpiau.ir/article-1-1085-en.html


Volume 8, Issue 30 (4-2018) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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