Volume 8, Issue 30 (4-2018)                   NCMBJ 2018, 8(30): 95-110 | Back to browse issues page

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Beiki A H. Molecular Sequence Analysis of the ITS rRNA Region for Identification of Fusarium species. NCMBJ. 2018; 8 (30) :95-110
URL: http://ncmbjpiau.ir/article-1-1097-en.html
Department of Biology, Faculty of Science, University of Qom, Qom, Iran , amirbeiki@gmail.com
Abstract:   (2245 Views)
Aim and Background:  Fusarium species are capable of infecting a wide range of crop plants. They can rarely cause human infections. Identification of Fusaria to the species level is necessary for biological, epidemiological, pathological, and toxicological purposes. The aim of this study was to evaluate phylogenetic relationships and finding specified repetitive patterns clustered in the area of ITS in order to identify different species of Fusarium.
Materials and Methods: Ribosomal DNA internal spacer region was amplified by polymerase chain reaction. ITS PCR product sequenced and analyzed by different methods.
Results: Amplification resulted in approximately 550 bp in all the isolates. In the phylogram derived from analysis of the ITS region, the Fusarium spp. examined were divided into ten groups
Discission: As expected the ITS region was variable and informative.
The high level of variation between internal transcribed spacer is concluded from the elimination or addition of repeating units is in this area.
Some strains are identified and some isolates definitely has a different pattern. There are repetitive patterns at the ITS in the particular species suggesting that this motifs can be used as a tool to identify species.
Keywords: Phylogenetic, Alignment, ITS
Full-Text [PDF 1277 kb]   (388 Downloads)    
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2018/07/18 | Accepted: 2018/07/18 | Published: 2018/07/18

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