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:: Volume 8, Issue 31 (7-2018) ::
NCMBJ 2018, 8(31): 49-56 Back to browse issues page
Analysis of the relationship between the frequency of class I integron and drug resistance in clinical isolates of Pseudomonas aeruginosa
Reza Yari , Mohammad Reza Mehrabi , Akram Mahdipour , Shahram Nakhjavan
Department of Biology, Faculty of Sciences, Borujerd Branch, Islamic Azad University, Borujerd, Iran , rezayari@yahoo.com
Abstract:   (1329 Views)
Aims and Background:
 Pseudomonas aeruginosais is one of the most important pathogens for nosocomial infections, particularly in ICU and burn. This bacterium causes diseases such as respiratory infections, sepsis and bacteremia. Since the serious infections caused by Pseudomonas resistance against conventional antibiotics is a serious problem and due to the placement of genes coding for antibiotic resistance on their Integrons and quick release feature identification of strains containing Integrons provide useful information on the extent and development of drug resistance. The aim of this study was to evaluate the frequency of class I integrons in Pseudomonas aeruginosa strains isolated from clinical samples and their relationship to antibiotic resistance.
Materials and Methods:
103 strains of Pseudomonas aeruginosa isolated from different clinical specimens were used. The drug susceptibility test, using 12 antimicrobial agents, was performed for all the isolates via agar disk diffusion method. The specific PCR was used to identify class I integrons and the relationship between class I integrons presence and antibiotic resistance was evaluated by χ2 test.
Results:
Analysis of antibiotic resistance patterns showed 75.7% of the isolates were resistant to cefotaxime and 73.7% of the isolates were sensitive to ceftazidime - clavulanic. The PCR results showed that 40.7% of isolates have class I integron. A significant correlation was obtained between the presence of class I integrons and resistance against most common antibiotics (P <0.05).
Conclusion:
Due to the high frequency of class I integrons and connection with drug resistance in Pseudomonas aeruginosa isolates, apply appropriate strategies for infection control and hospital treatment to prevent further spread is essential.
Keywords: Pseudomonas aeruginosa, antibiotic resistance, Integrons
Full-Text [PDF 432 kb]   (200 Downloads)    
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2018/09/5 | Accepted: 2018/09/5 | Published: 2018/09/5
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Yari R, Mehrabi M R, Mahdipour A, Nakhjavan S. Analysis of the relationship between the frequency of class I integron and drug resistance in clinical isolates of Pseudomonas aeruginosa. NCMBJ. 2018; 8 (31) :49-56
URL: http://ncmbjpiau.ir/article-1-1124-en.html


Volume 8, Issue 31 (7-2018) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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