Volume 8, Issue 31 (7-2018)
NCMBJ 2018, 8(31): 75-85 |
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Mokri F, Assmar M, Zarrabi S, Massiha A. The Evaluation of Antibacterial effect of Aquatic and Ethanolic Extract of Nerium oleander against Standard strains of Salmonella Typhi and Listeria Monocytogenes in vitro condition. NCMBJ 2018; 8 (31) :75-85
URL: http://ncmbjpiau.ir/article-1-1128-en.html
URL: http://ncmbjpiau.ir/article-1-1128-en.html
Department of Microbiology, Islamic Azad University, Lahijan Branch, Lahijan, Iran , mehdiassmar@gmail.com
Abstract: (4805 Views)
Aim and Background:
The Antibiotic resistance provides area to replace herbal treatment methods that have fewer side effects than common drugs. This study aimed to determine the antimicrobial effect of aquatic and ethanolic extracts of oleander (Nerium oleander) plant on the bacteria Salmonella Typhi and Listeria monocytogenes.
Material and methods:
In this experimental study, oleander plant leaves were gathered from Lahijan Plants Research Center located in Gilan province and after extracting, the Anti-bacterial effect of aquatic and ethanolic plant extracts was carefully evaluated against two strains of standard Salmonella Typhi PTCC 1609 and Listeria Monocytogenes PTCC 1295. And moreover, antimicrobial activity, minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) extracts, using serial dilution in broth and agar diffusion technique was determined.
Results:
In the method of diffusion in agar, all concentrations of oleander ethanol extract on Salmonella Typhi had deterrent effect. Yet, the deterrent effect of its ethanoic extract, compared to the aquatic extract is incomparably higher. The minimum inhibitory concentration of the ethanol extract 128 mg/ml and the minimum bactericidal concentration 256 mg/ml was subsequently determined. Aquatic and ethanol extracts of this plant were not proved to be effective on the standard strain of Listeria Monocytogenes.
Conclusion:
It seems that the ethanolic and aqueous extracts of oleander plant has a substantial antimicrobial effect on gram-negative bacterium Salmonella Typhi, under laboratory conditions. Therefore, they can be implemented more efficiently as pharmaceutical compounds or preservatives.
The Antibiotic resistance provides area to replace herbal treatment methods that have fewer side effects than common drugs. This study aimed to determine the antimicrobial effect of aquatic and ethanolic extracts of oleander (Nerium oleander) plant on the bacteria Salmonella Typhi and Listeria monocytogenes.
Material and methods:
In this experimental study, oleander plant leaves were gathered from Lahijan Plants Research Center located in Gilan province and after extracting, the Anti-bacterial effect of aquatic and ethanolic plant extracts was carefully evaluated against two strains of standard Salmonella Typhi PTCC 1609 and Listeria Monocytogenes PTCC 1295. And moreover, antimicrobial activity, minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) extracts, using serial dilution in broth and agar diffusion technique was determined.
Results:
In the method of diffusion in agar, all concentrations of oleander ethanol extract on Salmonella Typhi had deterrent effect. Yet, the deterrent effect of its ethanoic extract, compared to the aquatic extract is incomparably higher. The minimum inhibitory concentration of the ethanol extract 128 mg/ml and the minimum bactericidal concentration 256 mg/ml was subsequently determined. Aquatic and ethanol extracts of this plant were not proved to be effective on the standard strain of Listeria Monocytogenes.
Conclusion:
It seems that the ethanolic and aqueous extracts of oleander plant has a substantial antimicrobial effect on gram-negative bacterium Salmonella Typhi, under laboratory conditions. Therefore, they can be implemented more efficiently as pharmaceutical compounds or preservatives.
Keywords: Antimicrobial effect, aquatic and ethanolic extract, oleander plant, Salmonella Typhi, Listeria monocytogenes
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2018/09/8 | Accepted: 2018/09/8 | Published: 2018/09/8
Received: 2018/09/8 | Accepted: 2018/09/8 | Published: 2018/09/8
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