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:: Volume 9, Issue 33 (12-2018) ::
NCMBJ 2018, 9(33): 79-86 Back to browse issues page
Quantitative investigation of miRNA-196a-2 expression level in ovarian cancer patient in compare with normal by real Time PCR
Fahimeh Ezzatizadeh , Soyar Sari, Ardeshir Hesampour
Department of Molecular and cellular sciences, Faculty of advanced science & Technology, Tehran medical sciences, Islamic Azad university, Tehran, Iran
Abstract:   (852 Views)
Aim and Background: Current studies emphasize the key role of some non-coding RNAs in regulating the severity of different diseases as well as early diagnosis of various cancers. The aim of this study is to investigate the relation of ovarian cancer with miR-196a-2 expression level.
Material and method: In this study the expression of miR-196a-2 in 50 ovarian cancer patients and 50 control tissue sample were quantitatively investigated with quantitative reverse transcription PCR (qRT-pc) technique. The GAPDH gene was used as internal control gene.                
Result: Assessment of quantitative comparison showed that the miR-196a-2 expression level increased significantly in ovarian cancer patient in compare with control population. Increased miR-196a-2 probably effect through inhibiting the activity of tumor suppressor genes.
Conclusion: Based on miR-196a-2 expression level variation in tissue samples and quantitative increment in ovarian patients. We consider that evaluation of miR-196a-2 expressing level could be important role in investigation of ovarian cancer patients and could be use as biomarker to screen and early diagnosis of ovarian cancer patients.
Keywords: Ovarian epithelial cancer, miR-196a-2, qRT-PCR
Full-Text [PDF 514 kb]   (157 Downloads)    
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2018/12/22 | Accepted: 2018/12/22 | Published: 2018/12/22
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Ezzatizadeh F, Sari S, Hesampour A. Quantitative investigation of miRNA-196a-2 expression level in ovarian cancer patient in compare with normal by real Time PCR. NCMBJ. 2018; 9 (33) :79-86
URL: http://ncmbjpiau.ir/article-1-1162-en.html

Volume 9, Issue 33 (12-2018) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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