[Home ] [Archive]   [ فارسی ]  
:: Main :: About :: Current Issue :: Archive :: Search :: Submit :: Contact ::
:: Volume 9, Issue 33 (12-2018) ::
NCMBJ 2018, 9(33): 49-60 Back to browse issues page
Three-Rhizobia Screening On the amount of photosynthetic pigments of The Cowpea (Vigna Unguiculata) Plant Which Was Under Nickel Stress
Nazanin Moazzenpour , Sima Yahyaabadi , Monir Doudi
Department of Microbiology, Islamic Azad University , Falavarjan Branch, Isfahan, Iran , simayahyaabadi99@gmail.com
Abstract:   (628 Views)
Aim and Background:Bacteria types that live in the plants rhizosphere can improve the plants growth via taking advantage. In coexistence of leguminous plants with rhizobium bacteria, in addition to the fact that the main part of nitrogen is stabilized, the soil is also strengthened in terms of nitrogen. The purpose of this study was Three-Rhizobia Screening On the amount of photosynthetic pigments of The Cowpea (Vigna Unguiculata) Plant Which Was Under Nickel Stress.
 
Materials and methods: In this study, two standard samples of Rhizobium (PTCC 1654) (and (PTCC 1684) and strain isolated from the node were performed and PCR steps were performed on it and the strain as well as nickel was inoculated with clove bean plant.  To do so, the samples in three bacterial concentrations, namely 1.5 × 106 CFU/ml, 1.5 × 107 CFU/ml and 1.5 × 108 CFU/ml, and nickel in three concentrations of 200, 400 and 600 µM were inoculated. The samples were studied after ten days. The experiment was implemented in a factorial manner within the format of a completely randomized plot in three iterations. At the end of the period, the amount of the photosynthesis pigments was statistically analyzed by making use of SPSS software ver.19. Generally.
 
Results: The results showed that the concentration of inoculant strains isolated from node 1.5 × 107 CFU/ml and 200 mM nickel increased photosynthetic pigments (chlorophyll a, chlorophyll b, chlorophyll) in the plant.
 
Discussion and conclusion: Finally, it can be concluded that the use of growth promoting bacteria isolated from nodules on plant biochemistry of photosynthetic pigments cowpea (Vigna unguiculata) nickel was effective stress.
Keywords: Rhizobium, Nickel, Photosynthesis Pigments, PCR
Full-Text [PDF 609 kb]   (119 Downloads)    
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2018/12/22 | Accepted: 2018/12/22 | Published: 2018/12/22
Send email to the article author

Add your comments about this article
Your username or Email:

CAPTCHA code


XML   Persian Abstract   Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Moazzenpour N, Yahyaabadi S, Doudi M. Three-Rhizobia Screening On the amount of photosynthetic pigments of The Cowpea (Vigna Unguiculata) Plant Which Was Under Nickel Stress. NCMBJ. 2018; 9 (33) :49-60
URL: http://ncmbjpiau.ir/article-1-1163-en.html


Volume 9, Issue 33 (12-2018) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
Persian site map - English site map - Created in 0.06 seconds with 32 queries by YEKTAWEB 3855