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:: Volume 9, Issue 33 (12-2018) ::
NCMBJ 2018, 9(33): 0-0 Back to browse issues page
A survey of high-level resistance to gentamicin and aminoglycoside-modifying enzyme-encoding genes in Enterococcus faecalis and Enterococcus faecium isolated from infected burn wounds
Mohsen Mohamadi , Mohsen Jafari , Morteza Heidari , Hamid Eshaghi , Abazar Pournajaf , Ramin Kafshgari , Mehrdad Gholami
Abstract:   (280 Views)
Aim and Background: High-level resistance to gentamicin (HLGR) in enterococci diminish the synergistic effect of gentamicin and antibiotics affecting to the cell wall. Therefore, the aim of this study was to evaluate the High-level resistance to gentamicin and the genes encoding aminoglycoside modifying enzymes in Enterococcus faecalis and Enterococcus faecium isolated from burn wound infections.
Material and methods: In this cross-sectional study, a total of 182 samples were collected in a 9-month period from the Shahid-Motahari Hospital (Tehran). After identification of E. faecalis and E. faecium strains, an antibiotic susceptibility test was performed. A 120 μg gentamycin disks was used for detection of HLGR strains. PCR test was performed to identify the genes encoding AMEs.
Results: Of 62.6% Enterococcus strains, 64.1% and 35.9% were E. faecalis and E. faecium, respectively. 21.9% of E. faecium and 7.2% of E. faecalis were resistant to Vancomycin. Of 114 isolates of Enterococcus, 56.1% of strains were HLGR. All HLGR strains have the aac (6') -Ie -aph (2'') –Ia gene. 1.9% of the HLGR E. faecium strains carried the aph (2 ") -Id gene.
Conclusions: Our data showed that a high prevalence of resistance to gentamicin in burned wound infection in our hospital.  The main responsibility for the resistance to gentamicin in the strains isolated from studied burn patients is aac (6') -Ie -aph (2'') –Ia gene.
Keywords: Gentamicin, Aminoglycoside modifying enzymes, Enterococcus faecalis, Enterococcus faecium, Burn wound.
     
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2018/12/22 | Accepted: 2018/12/22 | Published: 2018/12/22
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Mohamadi M, Jafari M, Heidari M, Eshaghi H, Pournajaf A, Kafshgari R et al . A survey of high-level resistance to gentamicin and aminoglycoside-modifying enzyme-encoding genes in Enterococcus faecalis and Enterococcus faecium isolated from infected burn wounds. NCMBJ. 2018; 9 (33)
URL: http://ncmbjpiau.ir/article-1-1164-en.html


Volume 9, Issue 33 (12-2018) Back to browse issues page
مجله تازه های بیوتکنولوژی سلولی - مولکولی New Cellular and Molecular Biotechnology Journal
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