Materials and methods: AGS cells were transfused using lipofactamine solution and plasmid carrying the tagD gene encoding Helicobacter pylori or empty plasmid (control). RNA extraction was then performed from cultured cells and cDNA synthesis was performed, and then the eukaryotic expression of Helicobacter pylori gene tagD in AGS cells was investigated by RT-PCR method. The expression of GPR83 genes was evaluated by Real Time PCR method. It should be noted that the enoxin kit was used to evaluate apoptosis, and finally the expression of each gene was evaluated using SPSS software and t-test Indepent statistical tests.
Results: Results: The findings from gene expression analysis showed that the expression of GPR83 gene in AGS cells treated with tagD increased compared to control cells, but this increase in expression was not statistically significant (P = 0.0888).
Conclusion: Overall, the data obtained from this study showed that GPR83 gene expression is altered in cells treated with the Helicobacter pylori tagD gene and seems to play a role in the expression of Helicobacter pylori

Keywords: Helicobacter pylori, tagD, GPR83, AGS, IAU science.

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