Volume 10, Issue 40 (8-2020)
NCMBJ 2020, 10(40): 25-36 |
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Mokhtarnejad M, Pooryousef Miandoab M, Armeideh S. Inhibitory effects of ozonated grape seed oil on Staphylococcus aureus and Pseudomonas aeruginosa. NCMBJ 2020; 10 (40) :25-36
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URL: http://ncmbjpiau.ir/article-1-1315-en.html
Plant Protection Department, Faculty of Agriculture, Urmia University, Urmia, Iran.
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Inhibitory effects of ozonated grape seed oil on
Staphylococcus aureus and Pseudomonas aeruginosa
Mona Mokhtarnejad1, Mahmoud Pooryousef Miandoab1*, Shahram Armeideh2
1- Department of Biology, Faculty of Science, Urmia branch, Islamic Azad University, Urmia, Iran.
2- Department of Agroecology, Urmia Branch, Islamic Azad University, Urmia, Iran.
Abstract
Aim and Background: Due to the increasing resistance of pathogenic bacteria to common and newborn antibiotics, and effects of indiscriminate use of antibiotics on some organs of the body. Researchers are trying to find alternative drugs with plants, organic and physical sources.
The aim of this study was to evaluate the antibacterial effect of ozonated grape seed oil on Staphylococcus aureus and Pseudomonas aeruginosa.
Material and Methods: Grape seed oil by Soxhlet was extracted and ozonated, then the effect of ozonated grape seed oil using inhibitory growth diameter size and minimum inhibitory concentration and minimum bactericidal concentration indices on Staphylococcus aureus and Pseudomonas aeruginosa using analysis of variance (ANOVA) was evaluated and determined.
Results: Comparison of the diameter of the growth inhibitory zone with different treatments indicated that ozonated oil and ozonated water had high inhibitory effects (P<0.05).
The turbidity and clarity of ozone oil in microplate wells showed that the minimum inhibitory concentration (0.128 mg. ml-1) and the minimum bactericidal concentration (ineffective) on bacteria
Conclusion: According to the obtained results, grape seed oil can be used as an effective natural substitute for controlling these pathogens, especially S. aureus with proper safety.
Key words: Ozonated grape seed oil, Ozone, MIC, MBC
Staphylococcus aureus and Pseudomonas aeruginosa
Mona Mokhtarnejad1, Mahmoud Pooryousef Miandoab1*, Shahram Armeideh2
1- Department of Biology, Faculty of Science, Urmia branch, Islamic Azad University, Urmia, Iran.
2- Department of Agroecology, Urmia Branch, Islamic Azad University, Urmia, Iran.
Abstract
Aim and Background: Due to the increasing resistance of pathogenic bacteria to common and newborn antibiotics, and effects of indiscriminate use of antibiotics on some organs of the body. Researchers are trying to find alternative drugs with plants, organic and physical sources.
The aim of this study was to evaluate the antibacterial effect of ozonated grape seed oil on Staphylococcus aureus and Pseudomonas aeruginosa.
Material and Methods: Grape seed oil by Soxhlet was extracted and ozonated, then the effect of ozonated grape seed oil using inhibitory growth diameter size and minimum inhibitory concentration and minimum bactericidal concentration indices on Staphylococcus aureus and Pseudomonas aeruginosa using analysis of variance (ANOVA) was evaluated and determined.
Results: Comparison of the diameter of the growth inhibitory zone with different treatments indicated that ozonated oil and ozonated water had high inhibitory effects (P<0.05).
The turbidity and clarity of ozone oil in microplate wells showed that the minimum inhibitory concentration (0.128 mg. ml-1) and the minimum bactericidal concentration (ineffective) on bacteria
Conclusion: According to the obtained results, grape seed oil can be used as an effective natural substitute for controlling these pathogens, especially S. aureus with proper safety.
Key words: Ozonated grape seed oil, Ozone, MIC, MBC
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2020/10/14 | Accepted: 2020/08/31 | Published: 2020/08/31
Received: 2020/10/14 | Accepted: 2020/08/31 | Published: 2020/08/31
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