Volume 14, Issue 53 (12-2023)
NCMBJ 2023, 14(53): 43-58 |
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Zeinali Sehrig F, Zaefizadeh M, Ahmadizadeh C, Alivand R, Ghorbian S. Investigating the expression of miR-509-3-5p and miR-596 in tumor tissue and tumor peripheral tissue in breast cancer patients. NCMBJ 2023; 14 (53) :43-58
URL: http://ncmbjpiau.ir/article-1-1634-en.html
URL: http://ncmbjpiau.ir/article-1-1634-en.html
Islamic Azad University, Ardabil Branch, Ardabil, Iran , Zaefizadehmzaefi@gmail.com
Abstract: (491 Views)
Abstract
Aim and Background: Breast cancer is the most common type of cancer among women, which occurs in the epithelial tissue of the mammary gland. Various environmental, genetic and epigenetic factors play a role in the occurrence of breast cancer. According to the previous studies, one of the most important epigenetic changes involved in the occurrence of breast cancer is the dysregulationof the expression level of microRNAs. Therefore, the aim of this study was to investigate the expression level of miR-509-3-5p and miR-596 in breast cancer tumor tissue.
Material and methods:In the bioinformatics analysis, the datasets related to miRNAs (GSE40525 and GSE45666) were prepared from the GEO database. Data analysis was performed using the Affy package in R software. Sampling was done from 100 women with breast cancer and 100 control. Tissue RNA was extracted using Trizol solution. Then, using the cDNA synthesis kit, DNA was synthesized from the extracted RNAs, and the expression level of miR-509-3-5p and miR-596 was investigated using Realtime PCR method.
Results:According to the bioinformatics results, the expression level of miR-509-3-5p and miR-596 in breast cancer tissue reduced compared to healthy tissue. The expression level of miR-509-3-5p and miR-596 in tumor tissue was significantly lower than normal tissue (p<0.05).So these results confirmed the results of bioinformatics studies.
Conclusion: According to the results of the present study, which showed a decrease in the expression of miR-509-3-5p and miR-596 in breast cancer, it can be said that these miRNAs can be used as important diagnostic and therapeutic biomarkers in breast cancer.
Aim and Background: Breast cancer is the most common type of cancer among women, which occurs in the epithelial tissue of the mammary gland. Various environmental, genetic and epigenetic factors play a role in the occurrence of breast cancer. According to the previous studies, one of the most important epigenetic changes involved in the occurrence of breast cancer is the dysregulationof the expression level of microRNAs. Therefore, the aim of this study was to investigate the expression level of miR-509-3-5p and miR-596 in breast cancer tumor tissue.
Material and methods:In the bioinformatics analysis, the datasets related to miRNAs (GSE40525 and GSE45666) were prepared from the GEO database. Data analysis was performed using the Affy package in R software. Sampling was done from 100 women with breast cancer and 100 control. Tissue RNA was extracted using Trizol solution. Then, using the cDNA synthesis kit, DNA was synthesized from the extracted RNAs, and the expression level of miR-509-3-5p and miR-596 was investigated using Realtime PCR method.
Results:According to the bioinformatics results, the expression level of miR-509-3-5p and miR-596 in breast cancer tissue reduced compared to healthy tissue. The expression level of miR-509-3-5p and miR-596 in tumor tissue was significantly lower than normal tissue (p<0.05).So these results confirmed the results of bioinformatics studies.
Conclusion: According to the results of the present study, which showed a decrease in the expression of miR-509-3-5p and miR-596 in breast cancer, it can be said that these miRNAs can be used as important diagnostic and therapeutic biomarkers in breast cancer.
Type of Study: Research Article |
Subject:
Physiology
Received: 2024/02/3 | Accepted: 2023/12/22 | Published: 2023/12/22
Received: 2024/02/3 | Accepted: 2023/12/22 | Published: 2023/12/22
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