Volume 14, Issue 53 (12-2023)
NCMBJ 2023, 14(53): 59-70 |
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Siasi E, Aleyasin A. Association of rs737008 in PRM1 and rs4780356 in PRM2 Polymorphisms with Idiopathic Infertility in Iranian men. NCMBJ 2023; 14 (53) :59-70
URL: http://ncmbjpiau.ir/article-1-1645-en.html
URL: http://ncmbjpiau.ir/article-1-1645-en.html
Associate Professor, PhD of Getetic, Department of Microbiology, faculty of science, North Tehran Branch, Islamic Azad University, Tehran, Iran Associate Professor, PhD of Getetic, Department of Microbiology, faculty of science, North Tehran Branch, Islamic Azad University, Tehran, Iran Associate Professor, PhD of Getetic, Department of Microbiology, faculty of science, North Tehran Branch, Islamic Azad University, Tehran, Iran Associate Professor, PhD of Getetic, Department of Microbiology, faculty of science, North Tehran Branch, Islamic Azad University, Tehran, Iran Associate Professor, PhD of Getetic, Department of Microbiology, faculty of science, North Tehran Branch, Islamic Azad University, Tehran, Iran Associate Professor, PhD of Getetic, Department of Microbiology, faculty of science, North Tehran Branch, Islamic Azad University, Tehran, Iran Associate Professor, PhD of Getetic, Department of Microbiology, faculty of science, North Tehran Branch, Islamic Azad University, Tehran, Iran , emi_biotech2006@yahoo.ca
Abstract: (316 Views)
Aim and Background: Histones are replaced by protamines to package sperm head DNA during mammalian spermatogenesis. Protamine genes variation cause sperm DNA damage and is affect infertility in men. Therefore, this study aim was investigation on association of two rs737008 in PRM1 gene and rs4780356 in PRM2 gene polymorphisms with azoospermia and oligospermia in Iranian idiopathic infertile men.
Material and Methods: DNA extraction from blood samples of 96 idiopathic infertile men with azoospermia and oligospermia and 100 normal control men. Prevalance of two studied polymorphisms was identified by restriction fragment length polymorphism (PCR-RFLP). Then results were confirmed with sequencing.
Results: A mutant allele frequency of rs737008 in PRM1 gene polymorphism was difference in patient and control groups but statistical analysis showed no significant association between this polymorphism prevalance among case and control groups (P>0.05). By genotyping shown no existent rs4780356 in PRM2 gene polymorphism in anyone of case and control groups.
Conclusion: This study finding indicated there was not association with prevalence of two rs737008 in PRM1 gene and rs4780356 in PRM2 gene polymorphisms with oligospermia and azospermia and idiopatic male infertility in Iranian population. More studies need to demonstrate the role of these two polymorphisms with idiopathic infertility in Iranian men.
Material and Methods: DNA extraction from blood samples of 96 idiopathic infertile men with azoospermia and oligospermia and 100 normal control men. Prevalance of two studied polymorphisms was identified by restriction fragment length polymorphism (PCR-RFLP). Then results were confirmed with sequencing.
Results: A mutant allele frequency of rs737008 in PRM1 gene polymorphism was difference in patient and control groups but statistical analysis showed no significant association between this polymorphism prevalance among case and control groups (P>0.05). By genotyping shown no existent rs4780356 in PRM2 gene polymorphism in anyone of case and control groups.
Conclusion: This study finding indicated there was not association with prevalence of two rs737008 in PRM1 gene and rs4780356 in PRM2 gene polymorphisms with oligospermia and azospermia and idiopatic male infertility in Iranian population. More studies need to demonstrate the role of these two polymorphisms with idiopathic infertility in Iranian men.
Type of Study: Research Article |
Subject:
Physiology
Received: 2024/03/5 | Accepted: 2023/12/22 | Published: 2023/12/22
Received: 2024/03/5 | Accepted: 2023/12/22 | Published: 2023/12/22
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