Aim and Background: Phytic acid is an important mineral compound which can be preserved in plants. Phytase (E.C. 3.1.3.26 or E.C. 3.1.3.8) has been used as a cereal feed additive that can enhance the phosphorus and mineral absorption in monogastric animals food so it can reduce the phosphorus output into the environment. In this research, Cloning of gene encoding phytase containing hexahistidine-tag was conducted in Escherichia coli and then yeast for purification of the produced enzyme after expression in this yeast.
Material and method: Sequence of protein encoding Peniophora lycii fungal phytase, was obtained from protein data bank, designed and then synthesized according to the expression codon usage of the yeast. A piece of DNA containing hexahistidine-tag was added into the pFPMTMF 14