Aim and background:Based on confirmed reports, it is clear that breast cancer is one of the most prevalent diseases among women.Hydroxyureais one of the important drugs used in chemotherapy.despite its therapeutic propertie, this drug has many adverse effects in long-term, this study was performed in order to use the Nano drug delivery technologyusing liposomes drug deliveryfor reducingthe adverse effects and increasing therapeutic index as well.

Materials and Methods:A certain ratio of Phosphatidylinositol choline and cholesterol was synthesized in order to prepare the nanoliposome followed by adding the hydroxyurea drug. The mean diameter of nanoliposomal hydroxyurea was determined by zeta sizer system. The percent of drug released from liposome was performed by dialysis. Also, encapsulation efficiency of nanoliposomal hydroxyurea was calculated.Ultimately the cytotoxicity of formulation was probed by MTT assay.

Results:The mean diameter of nanoliposome hydroxyurea and also and its encapsulation efficiency were recorded 402.5 nanometers and 70.836 % respectively. The pattern of drug release from nanoliposomes using dialysis method showed that the drug release of nanoliposome drug within 28 hours was equal to 25.85 %.

Conclusion:This study showed that the cytotoxicity effect of nanoliposomal drug is more than that of the released drug.