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ghiasi M, shahhosseiny M H, mohajerani H R. توسعه یک روش PCR جهت تشخیص آلودگی های مایکوپلاسما در رده های سلولی و فراورده های بیولوژیکی. NCMBJ 2014; 4 (14) :41-46
URL: http://ncmbjpiau.ir/article-1-516-en.html

Aim and Background: Contamination of cell linesandbiological productsisone of the major problemsof cell culturetechniques. Rapiddetection ofMycoplasmacontaminationincell cultureisan importantpartof controlledlaboratory, and shouldthere bea methodinlaboratorycell cultures. Infectedcells,leading tounreliableresults, so the need for areliablemethod forlaboratoriesisessential. Polymerase chain reaction, a technique ofrapid, sensitive andspecificdetection ofbacteriais considered. The aim ofthisstudy was to evaluatethe efficacy ofPCRin the detectionofpollutants incell culturesand otherbiologicalproducts.

Materials and Methods: Inthisstudy ,PCR techniques usingprimers MGSO and GPO-1andtargetgene 16SrRNA was optimized. AlsousedPCRmethodwas evaluatedin terms ofsensitivity and specificity. Finally,a simpleDNAextractionandPCRof 72celllinestested.