Volume 4, Issue 15 (9-2014)
NCMBJ 2014, 4(15): 29-34 |
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Attaran Fariman G, Moosavipoor Y, shakori A. Phylogenetic study of Peronia peronii species (Mollusca Gastropod: sea slug) in inter-tidal Chabahar coast, based on 18s-rDNA sequence. NCMBJ 2014; 4 (15) :29-34
URL: http://ncmbjpiau.ir/article-1-549-en.html
URL: http://ncmbjpiau.ir/article-1-549-en.html
Marine and Maritime Sciences Faculty of Chabahar, Department of Biology, Faculty of Marine Sciences, Chabahar Maritime University of Marine Sciences , Chabahar, Iran , gilan.attaran@gmail.com
Abstract: (10526 Views)
Aim and Background: Phylogenetic analysis and morphological identification on species of each region can be used as the basis for establishing subsequent studies. Many phylogenetic studies have been carried out according to mitochondrial genome on gastropods group in other coastal areas of the world but there are not much data on nuclear genome. Most Gastropod study in Iran is founded on morphology of the species, and there are no records of molecular study from south coast of Iran. In this study phylogenetic analysis of a sea slug species from Chabahar coast was examined for the first time.
Materials and Methods: Samples were collected from rocky shores of Tis in Chabahar and transferred to the laboratory. The samples were analyzed morphologicaly prior to being frozen in freezer -80 degrees. DNA was extracted by using CTAB method and then Polymerase Chain Reaction, sequencing alignment were conducted and phylogenic tree was obtained.
Results: Nucleotides sequence of the gene in 18s rDNA region was analyzed. The phylogenetic analysis was conducted and found that the Iranian species and other species in Panpulmonata clad have more divergence than species of the other two clads.
Conclusion : Maximum likelihood phylogenetic analysis showed that the Iranian species from Chabahar coast is in the Panpulmonata clad and with 100% bootstrap support is a sister group Peronia Cf.peroni. The molecular results are confirmed by morphological features.
Type of Study: Research Article |
Subject:
Genetics
Received: 2014/09/22 | Accepted: 2014/09/22 | Published: 2014/09/22
Received: 2014/09/22 | Accepted: 2014/09/22 | Published: 2014/09/22
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