Volume 4, Issue 15 (9-2014)                   NCMBJ 2014, 4(15): 61-65 | Back to browse issues page

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Jafarinejad F, Asadi Tehrani G, Aminbakhsh M, Kazemi B, Yasaee V R, Yarian F, et al . Cloning and Expression of Influenza H1N1 virus M2 protein in BL21 (DE3) E.coli. NCMBJ 2014; 4 (15) :61-65
URL: http://ncmbjpiau.ir/article-1-554-en.html
3- Cellular & Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran , m.bandehpour@sbmu.ac.ir
Abstract:   (12952 Views)
Aim and Background: Influenza A is a major cause of mortality in a year. There has been an efficient vaccine available against it since 1960. Antigenic variation is a preventive agent for yearly vaccine production. Now researchers focus on the constant antigens of viruses. M2 protein makes an ionic channel in the coat of influenza, a virus that is effective on infection by virus. This protein is conserved and it is considered as a proper candidate vaccine for influenza infection. 
Materials and Methods: Due to limitations for influenza virus culture at the lab, the sequence of influenza virus H1N1 strain of Iran was synthesized. It was sub-cloned into pET22b vector recombinant protein and expressed in BL21 E.coli and confirmed with specific antibody by SDS-PAGE and western blot techniques. 
Results: The concentration of recombinant M2 protein was 300μg/ml confirmed by specific antibody. 
Conclusion: Influenza M2 protein could be expressed in BL21 E.coli host.
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Type of Study: Research Article | Subject: Genetics
Received: 2014/09/27 | Accepted: 2014/09/27 | Published: 2014/09/27

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