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Aims . CEL I, is isolated from celery and the first eukaryotic nuclease is known to have cleavage DNA with high specificity at sites of the two DNA strands in a heteroduplex DNA molecule . The aim of this study was cloning, expression and purification CEL I endonuclease and a simple method for the detection of mutation s.

Material & Methods . CEL I mRNA was used for RT-PCR. CEL I cDNA was synthesized and cloned into T-vector pTZ57R, then CEL I subcloned into pET32a expression vector. Recombinant plasmid was transformed into BL2I bacterial cells. Expression of recombinant plasmid was analyzed by western blot technique. Protein was purified by affinity chromatography . In first study, Recombinant enzyme was active in mutation detection in Product PCR normal and mutant BRCA1 gene.

Results. The recombinant ( pET32a /CEL I) was successfully expressed in BL2I cells. Western blot analysis showed that the successfully expressed CEL I in the cells transfected . The first study showed that recombinant enzyme is kno wn to nick mismatch sites of the two DNA strands in a heteroduplex DNA molecule.

Conclusion. The expression of CEL I in BL2I cells was strong. In vitro, purification protein was successful. Recombinant nucleases CEL I known to nick mismatch sites in a heteroduplex DNA molecule.

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Aims and Background. Human serum albumin (HSA) is the most important components of the circulatory system. HSA can transport many metabolites and hormones in the body . It is effective for blood volume stabilization and the burn treatment. The preparation of HSA from human plasma is costly and the risk of contamination with various pathogens is high. Therefore, biotechnology could promise a new way to solve the problems.

Materials and methods. HSA gene was synthesized based on the sequence of the gene in NCBI website . HSA was cloned into pET 22 b expression vector and then t he recombinant plasmid was transformed into BL21 (DE3) competent cell . The colony (containing recombinant expression vector) was cultured overnight. The culture was induced with IPTG 1/5 mM in the logarithmic phase (OD₆₀₀=0.6). Sampling was carried out in different times and it was analyzed on %10 (w/v) SDS-PAGE. The expression of recombinant human serum albumin (rHSA) was confirmed by western blot. Finally, rHSA was Alpha purified by affinity chromatography.

Results.In this study, human serum albumin was produced in the bacterial periplasmic environment.  The highest expression level of rHSA was observed 24 h after the bacterial induction.

Conclusion.  In this study, rHSA was produced in E.coli host.

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Aim and Background: Influenza A is a major cause of mortality in a year. There has been an efficient vaccine available against it since 1960. Antigenic variation is a preventive agent for yearly vaccine production. Now researchers focus on the constant antigens of viruses. M2 protein makes an ionic channel in the coat of influenza, a virus that is effective on infection by virus. This protein is conserved and it is considered as a proper candidate vaccine for influenza infection. 

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Resistance to anti-cancer therapy in patients with advanced solid tumors reveals the need for new treatments.  Major challenges of Anti-cancer therapy is, specificity of treatment. Treatment will be ideal if cancer cells be eradicated without side effects on normal cell.

 Bacteria are used in different ways to treatment of cancer, either to provide direct tumoricidal effects or to deliver tumoricidal molecules .there are two ways to use bacteria as vectors, included, tumor-specific bacterial replication and  intracellular plasmid transfer. On the other hand, Bacterial toxins act by killing the cells and control of cellular processes for example cell proliferation, differentiation and apoptosis that they are associated with cancer. Spores are very resistant bacteria that survive even in oxygen-rich conditions, but, they are not able to grow. In conditions tumor the spore grow and germinate without affecting on normal cells. Bacteria and immune system: bacterial DNA vaccines apply immune system against cancer cells via cancer antigen Presentation. Here, we review the Different applications of bacterial therapy, mechanisms of action and successes at preclinical and clinical levels. Further developments in these studies will add a new dimension to cancer treatment.

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Aim and Background: Today, many patients with coagulation disorders due to defective or non-functioning coagulation factors and the use of various blood thinners need to check their prothrombin total and quantify determination of direct thrombin inhibitors. Despite the study of the ecarin from Echis carinatus venom as a direct activator of prothrombin, there have been not investigations into the active site of this enzyme.
Material and methods: Ecarin metalloproteinase domain (639 bp) was synthesized into the pcDNA3.1. Recombinant plasmid was transfected into HEK293 cell line. Protein expression was evaluated using SDS-PAGE and its function in reaction with prothrombin and using a prothrombin time test was measured.
Results: The results showed that the metalloproteinase domain of ecarin produced in the HEK293, like whole ecarin, was able to activate prothrombin to thrombin; but its activity was slow than one produced in the prokaryotic system.
Conclusion: In this study, the recombinant ecarin metalloproteinase produced in HEK293 cells, like whole ecarin, was able to activate prothrombin to thrombin and was introduced for the first time as a suitable alternative to both natural and recombinant ecarin in methods based on prothrombin to thrombin conversion.

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Aim and Background: Rheumatoid arthritis(RA) is a complex and chronic disease of joint inflammation. Environmental factors that are important in genetically predisposed people are involved in the occurrence of the disease and its progress. This disease has a lot to related to genetics, including human class 2 antigens. In various studies, this relation is about 60%. This relationship is more positive in people with Anti-citrullinated protein antibodies (ACPA) than negative ones. The aim of this study is to investigate the relationship between cytokines and soluble proteins in the body of a patient with Rheumatoid arthritis before symptoms appear.
Materials and Methods: In this research, analysis with Enrichr, WebGestalt, and GeneMANIA was used to find genes related to the disease. In order to check the amount of cytokines predicted, ELISA and Real time RT-PCR methods were applied. Results: By examining the System Biology methods, the candidate genes included IL-6R, IL-6 and IL-23A. The samples were taken from 46 RA patients referred to the rheumatology department of the Babol hospital, and a significant decrease (Pv=0.1255) of IL-6 was observed in patients undergoing treatment and a significant increase (Pv=0.4414) in the amount of this cytokine receptor in the serum compared to healthy individuals. While the expression of IL6 and IL6R genes increased in the patient group compared to the healthy group.
Conclusion: Cytokine IL-6 as an inflammatory cytokine in treated patients decreased in serum and showed a significant increase in RNA level.