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Showing 3 results for Noroozi

Nahid Sadeghi, Hassan Khalaji, Maryam Noroozian, Pouneh Mokhtari,
Volume 3, Issue 11 (9-2013)
Abstract

Aim and Background: The world is aging. The increase of lifetime and number of elderly people causes some concerns about the future and inevitable problems that will exist for this particular period of time. Alzheimer is a progressive disease, which reaches more advanced stages in case of non-intervention. Some of the methods including physical and mental activities have shown that they can be effective in preventing and slowing disease progression. The objective of this study is also verifying the effect of physical activities on the memory function level of the 50-70 year-old-women who are affected by memory impairment.

Materials and Methods: 16 female subjects (mean age 61/1 ± 6/5) with MCI = 0.5 (mild cognitive impairment), illiterate, healthy (Based on the medical view for participation in physical activities) and without regular physical activities in Tehranelderly nursing home were selected. The randomly practices intervening: experimental group was thephysical activities and the control group (control) wasthe one without any activities. (Each group consisted of eight persons). Duration of intervening was three months, three sessions per week and each session lasted 60 minutes. For the diagnosis of dementia, the Clinical Dementia Rating (CDR) was used. Also in order to control the depression level, before and after implementation of the plan, the Geriatric Dementia Scale (GDS) was carried out. To evaluate the data,paired t-test, ANCOVA analysis method and the Bonferroni post hoc were used.

Results: The results indicated that intervening method(physical exercise) had significanteffect onthe memory impairment in increasing the memory level of the 50-70 year-old-women with MCI. Being affected for subscales was the memory subscale. In this consideration, no changes were observed in the depression level and then the level of depression has not interfered in the function level of the participators.

Conclusions: In this study, interventional procedure including physicalexercise caused an improvement in the memory of 50-70-year-old women with mild cognitive impairment. Therefore, doing the physical performance in patients with mild cognitive impairment is effective.


Tayebeh Hassan Soltan, Mostafa Noroozi, Mohammad Ali Amoozegar,
Volume 6, Issue 24 (10-2016)
Abstract

Aims & Background: Due to Micro algae properties including nutritional value, medicinal properties, high amount of protein, vitamins, minerals, natural pigments such as phycocyanin and Carotenoids, they can use in the food industry - hygienic, cosmetics, food additives for human, animals, poultry and Aqua feed. Carotenoids are an important group of natural pigments, which produce only by plants and micro algae; in addition they have also antioxidant properties which make them useful for medical applications. This study was conducted to identify the micro algal species by standard techniques such as morphological and biochemical characteristics

Materials and methods: In this study, the samples were isolated and purified from Golestan coasts, Khazar Lake and cultivated in F/2 & BBM medium. The following method was used for measurements: Methanol extraction Lichtenthaler for total carotenoids and chlorophyll and DPPH for antioxidant activity.

Results: The purpose of this study was to investigate the potential locally Micro algae from Golestan coasts as a Carotenoids Source. Morphological characters identification has confirmed, Selenastrum sp. Chlorella volgaris , Chlamydomona debaryana ,Chlorella sorkinia as isolated species. Their Chlorophyll a concentration were 6.7, 5.1, 12.5, 7.8 and concentration total Carotenoid 2.2, 1.4, 2.4, 1.6 mg/gr total respectively. IC50 of isolates methanol extract were 2.87, 4.03, 2.78, 3.75 mg and IC50 of isolates hexan extract were 6.49, 4.9, 3.75, 8.35mg.

Conclusion: There is a high diversity of microalgae in Iran with unknown applied potential. Thus in this study we have isolated four strains and their characters have investigated. Based on the results antioxidant activity of methanol extract is upper than anti oxidant activity of hexan extract. The highest anti oxidant activity was seen in Chlamydomona debaryana.    


Najmeh Yarkeh Salkhori, Taghi Naserpour Farivar, Jamileh Noroozi, Reza Najafipour, Parviz Pakzad, Safarali Alizadeh,
Volume 14, Issue 53 (12-2023)
Abstract

Aim and Background: Oncolytic viral therapy is known as promising approach for cancer treatment. Oncolytic viruses like adenoviruses can proliferate in cancerous cell and disintegrated these cells.  Serotype 5 adenovirus attached on surface of host cell via coxsackie-adenovirus receptors. The expression rate of this receptor is highly variable in surface of cancerous cells. The aim of the present study was production of an oncolytic adenoviruses with high selectivity feature for gastric adenocarcinoma cells (AGS) that can proliferate in this cell and destroy them.
Material and methods: In this experimental study, the octreotate sequence was cloned into HI-loop of the adenovirus fiber. Thus, the pAd5TATE (wt) and pAd5TATE (ΔE1, ΔE3) recombinant vectors were constructed. pAdZ-TATE (wt) vector was synthesized using pADZ cloning system. Ad5TATE (wt) and Ad5TATE (ΔE1, ΔE3) recombinant viral particles were synthesized and propagated in HEK293 cells. Using the calcium phosphate procedure, the HEK293 cell line was transfected with the recombinant vectors and the new virus particles were produced. The AGS cell line was transfected by the Ad5 (wt) and Ad5TATE (wt) and the cell destruction of these cells were investigated in different times. Using the MTT test the cell surveillance rate was evaluated in AGS cell line that treated with recombinant vector. Cell destruction effects (CPE) started in less than 18 hours and were completed 72 hours after infection.
Results: The titer of recombinant viruses was calculated by the TCID50 method as 107 (for 100 Lµ of viral solution). Also, Ad5TATE (ΔE1, ΔE3) virus titer was 107 TCID50/ml by the TCID50 method. The results showed that Ad5TATE (wt) could infect AGS cells compared to Ad5(wt). The results of the MTT test showed that with the increase in the concentration of viral particles and storage time, the number of live AGS cells decreased significantly.
Conclusion: The findings of this research showed that the Ad5TATE (wt) recombinant virus could specifically infect gastric adenocarcinoma (AGS) cancer cells and destroy them after replication. Therefore, the Ad5TATE (wt) virus can be used as an oncolytic agent against gastric adenocarcinoma (AGS) cancer cells.

 

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