RT - Journal Article T1 - The Evaluation of Antibacterial effect of Aquatic and Ethanolic Extract of Nerium oleander against Standard strains of Salmonella Typhi and Listeria Monocytogenes in vitro condition JF - NCMBJ YR - 2018 JO - NCMBJ VO - 8 IS - 31 UR - http://ncmbjpiau.ir/article-1-1128-en.html SP - 75 EP - 85 K1 - Antimicrobial effect K1 - aquatic and ethanolic extract K1 - oleander plant K1 - Salmonella Typhi K1 - Listeria monocytogenes AB - Aim and Background: The Antibiotic resistance provides area to replace herbal treatment methods that have fewer side effects than common drugs. This study aimed to determine the antimicrobial effect of aquatic and ethanolic extracts of oleander (Nerium oleander) plant on the bacteria Salmonella Typhi and Listeria monocytogenes. Material and methods: In this experimental study, oleander plant leaves were gathered from Lahijan Plants Research Center located in Gilan province and after extracting, the Anti-bacterial effect of aquatic and ethanolic plant extracts was carefully evaluated against two strains of standard Salmonella Typhi PTCC 1609 and Listeria Monocytogenes PTCC 1295. And moreover, antimicrobial activity, minimum inhibition concentration (MIC) and minimum bactericidal concentration (MBC) extracts, using serial dilution in broth and agar diffusion technique was determined. Results: In the method of diffusion in agar, all concentrations of oleander ethanol extract on Salmonella Typhi had deterrent effect. Yet, the deterrent effect of its ethanoic extract, compared to the aquatic extract is incomparably higher. The minimum inhibitory concentration of the ethanol extract 128 mg/ml and the minimum bactericidal concentration 256 mg/ml was subsequently determined. Aquatic and ethanol extracts of this plant were not proved to be effective on the standard strain of Listeria Monocytogenes. Conclusion: It seems that the ethanolic and aqueous extracts of oleander plant has a substantial antimicrobial effect on gram-negative bacterium Salmonella Typhi, under laboratory conditions. Therefore, they can be implemented more efficiently as pharmaceutical compounds or preservatives. LA eng UL http://ncmbjpiau.ir/article-1-1128-en.html M3 ER -