TY - JOUR T1 - Inhibitory effects of ozonated grape seed oil on Staphylococcus aureus and Pseudomonas aeruginosa TT - بررسی اثر ضد باکتریایی روغن هسته انگور ازونه روی باکتری استافیلوکوکوس اورئوس و سودوموناس آئروژینوز JF - NCMBJ JO - NCMBJ VL - 10 IS - 40 UR - http://ncmbjpiau.ir/article-1-1315-en.html Y1 - 2020 SP - 25 EP - 36 KW - Ozonated grape seed oil KW - Ozone KW - MIC KW - MBC KW - IAU science. N2 - Aim and Background: Due to the increasing resistance of pathogenic bacteria to common and newborn antibiotics, and effects of indiscriminate use of antibiotics on some organs of the body. Researchers are trying to find alternative drugs with plants, organic and physical sources. The aim of this study was to evaluate the antibacterial effect of ozonated grape seed oil on Staphylococcus aureus and Pseudomonas aeruginosa. Material and Methods: Grape seed oil by Soxhlet was extracted and ozonated, then the effect of ozonated grape seed oil using inhibitory growth diameter size and minimum inhibitory concentration and minimum bactericidal concentration indices on Staphylococcus aureus and Pseudomonas aeruginosa using analysis of variance (ANOVA) was evaluated and determined. Results: Comparison of the diameter of the growth inhibitory zone with different treatments indicated that ozonated oil and ozonated water had high inhibitory effects (P<0.05). The turbidity and clarity of ozone oil in microplate wells showed that the minimum inhibitory concentration (0.128 mg. ml-1) and the minimum bactericidal concentration (ineffective) on bacteria Conclusion: According to the obtained results, grape seed oil can be used as an effective natural substitute for controlling these pathogens, especially S. aureus with proper safety. M3 ER -