TY - JOUR T1 - Investigation of let-7 family expression in CD4+ T cells induced by anti-CD2, CD3, CD28 and Interleukin-2 and identifying their potential targets TT - بررسی بیان microRNA های خانوادهlet-7 در سلول های CD+4 T تحت القاء آنتی بادی های C‏D2,CD3,CD28 و اینترلوکین 2 و تعیین اهداف احتمالی آن ها JF - NCMBJ JO - NCMBJ VL - 3 IS - 10 UR - http://ncmbjpiau.ir/article-1-328-en.html Y1 - 2013 SP - 15 EP - 20 KW - microRNA KW - Interleukin-2 KW - anti-CD2 KW - CD3 KW - CD28 KW - let-7 family KW - clonal expansion N2 - Aime and Background. The aim of this study was to evaluate let-7 family members expression in activated naïve CD4+ T cells and IL-2 induced cells. Let-7 family has oncogenic and tuomor suppressive activity in different cells. Methods. Naïve CD4+ T cells were activated by anti-CD2, CD3, CD28 microbeads. After 3 days, the activated cells stimulated by IL-2(0.75ng/ml). after 72h total RNA extracted the cells and c-DNA synthesis and Q-RT-PCR performed by miRCURY LNATM universal RT miRNA PCR kit. miRNA expression analyzed by geneX software and ∆∆Ct method. Results. Five members of let-7 family over-expressed in activated cells compared with IL-2 induced cells. Targets Predicted by miRDB and miRanda software including families of DUSP, PTPN and SOCS that two first families inhibit TCR signaling and latest suppress JAK/STAT pathway. Conclusion. It appears that let-7 family play important role in clonal expansion of activated CD4+ T cells by specific genes suppression in post-transcriptional levels for appropriated immune response. M3 ER -