fa بررسی ارتباط بین پلی‌مورفیسم D/I ژن آنزیم مبدل آنژیوتانسین با چاقی در جمعیت استان اردبیل ژنتیک Genetics مقاله پژوهشی Research Article <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">سابقه</span></span></strong> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">وهدف</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> امروزه ژن&shy;های زیادی که تأثیرهای پیچیده&shy;ای بر چاقی دارند گزارش شده است و یکی از این ژن&shy;ها آنزیم مبدل آنژیوتانسین (</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">ACE</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) است که عامل مؤثر در متابولیک بافت چربی می&shy;باشد و در رشد و عملکرد سلول&shy;های آدپیوسیت درگیر می&shy;باشد. هدف از این مطالعه، بررسی ارتباط بین این پلی&shy;مورفیسم با چاقی در جمعیت استان اردبیل بود.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">مواد و روش&shy;ها</span></span></strong>: <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دراین بررسی 172 نفر بر اساس شاخص توده بدنی به دو گروه افراد </span></span> &nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;و افراد چاق با&nbsp; </span></span> &nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تقسیم شدند و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">DNA</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نمونه ها با استفاده از روش نمک اشباع استخراج شده و ژنوتیپ افراد تعیین گردید. </span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">یافته&shy;ها</span></span></strong>: <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">درگروه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، 5/57% مردو 5/42% زن وگروه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 42% مردو 58% زن بودند. میانگین سنی افراد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">،5/47 سال و</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">میانگین سنی افراد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">5/52 سال بود. میانگین </span></span>&nbsp;<span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">BMI</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">افراد </span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;، 19/25 و میانگین </span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">BMI</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> افراد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، 58/32 می باشد</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">بحث:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> فراوانی ژنوتیپ</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">DD</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">افراد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">چاق با</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، 83/0 و در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">افراد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">71/0بود</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">که این اختلاف ازنظرآماری معنی دارمی&shy;باشد (044/0 ).</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">نتیجه&shy;گیری</span></span></strong>: <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یافته های حاصل از این تحقیق نشان داد که ژنوتیپ </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">DD</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در ژن </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">ACE</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> می تواند با چاقی جمعیت استان اردبیل ارتباط داشته باشد</span></span><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">. </span></span> <strong>Aim and Background:</strong><br> Today many genes have been reported that affect obesity in complex ways. One of these genes is the polymorphism of ACE gene. Angiotensin converting enzyme (ACE) is an important factor that can affect adipose tissue metabolism. The aim of this study was to investigate the relationship between ACE gene polymorphism and obesity in the population of Ardabil province<span dir="RTL">.</span><br> <strong>Methods and materials:</strong><br> The 172 people attending this study were divided into two individuals of BMI < 30 and BMI&ge; 30 according to their body mass index.&nbsp; The DNAs of the sample were extracted using the saturated salt method and the extracted strains from normal and obsess participants were compared with each other under electrophoresis by PCR.<br> <strong>Results:</strong><br> In the group by BMI<30 there were 57.5% male and 42.5% female participants. By contrast, in the group by BMI&ge;30, there were 42% male and 58% female participants. The average age of the participants in BMI<30 group was 47.55 years but in the BMI&ge;30 group it was 52.45 years. BMI mean of the people in BMI<30 was 25.19 and BMI mean of the people in BMI&ge;30 was 32.58. DD genotype frequency in BMI&ge;30 was 0.83 which is more than the frequency of BMI<30 (0.83 against 0.71).<br> <strong>Conclusion:</strong><br> The obtained results of this research suggested that genotype DD in ACE gene has a relationship with the obesity of Ardabil province population.<br> &nbsp; چاقی, پلی‌مورفیسم ژن ACE, آنزیم مبدل آنژیوتانسی Obesity, polymorphism of ACE gene, Angiotensin-Converting Enzyme (ACE) 19 24 http://ncmbjpiau.ir/browse.php?a_code=A-10-1108-2&slc_lang=fa&sid=1 Fariba Oji فریبا اوجی 10031947532846007695 10031947532846007695 No Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran گروه زیست شناسی، دانشگاه آزاد اسلامی واحد اردبیل، اردبیل، ایران Hashem Yaghoubi هاشم یعقوبی yaghoubi_h@iauardabil.ac.ir 10031947532846007696 10031947532846007696 Yes Department of Biology, Ardabil Branch, Islamic Azad University, Ardabil, Iran گروه زیست شناسی، دانشگاه آزاد اسلامی واحد اردبیل، اردبیل، ایران