fa بررسی ارتباط بین پلی‌مورفیسم عملکردی ژن FSHR و میزان ابتلا به ناباروری در زنان ایرانی ژنتیک Genetics مقاله پژوهشی Research Article <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">سابقه و هدف</span></span></strong><strong><span style="font-family:b lotus;"><span style="font-size:16.0pt;"> :</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> پلی&shy;مورفیسم&shy;های موجود در ژن</span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">FSHR </span></span></em>&nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;درپاسخ تخمدان به هورمون تأثیر گذارند. لذا بررسی پلی&shy;مورفیسم مربوط به جایگاه&nbsp; 680 موجود در ژن</span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">FSHR</span></span></em> &nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در زنان تحت درمان با روش </span></span>&nbsp;<span dir="LTR"><span style="font-family:times new roman,serif;">IVF</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">(موفق یا ناموفق) امری مهم است</span></span><span style="font-family:b lotus;"><span style="font-size:12.0pt;">.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">مواد و روش</span></span></strong><strong><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:16.0pt;">&shy;</span></span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">ها</span></span></strong><span style="font-family:b lotus;"><span style="font-size:16.0pt;"> :</span></span><span style="font-family:b lotus;"><span style="font-size:12.0pt;"> از </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">31 زن نابارور دارای حداقل یک </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">IVF</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ناموفق و30 زن دارای حداقل 1 بارداری موفق استخراج </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">DNA </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;به عمل آمد و سپس با استفاده از تکنیک</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">( </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">PCR</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ) و </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">(Sequencing</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">)</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> برای پلی</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مورفیسم&nbsp; موجود در جایگاه 680 مورد بررسی قرار گرفتند.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">یافته&shy; ها</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">:</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مقدار کل گنادوتروپین تزریق شده در زنان نابارور با ژنوتیپ </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Ser680</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بیش&shy;تر از زنان نابارور با ژنوتیپ</span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Asn680 </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;&nbsp;بود. تجزیه و تحلیل نشان داد پاسخ تخمدان&nbsp; به هورمون </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">FSH</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در ژنوتیپ&nbsp; </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Ser680</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در ژن </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">FSHR</span></span></em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ضعیف&shy;تر از ژنوتیپ </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Asn680</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در زنان نابارور است.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">بحث</span></span></strong><span style="font-family:b lotus;"><span style="font-size:16.0pt;">:</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این مطالعه &nbsp;ارتباط معنی</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">&shy;</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دار پلی&shy;مورفیسم ژن </span></span><em><span dir="LTR"><span style="font-family:times new roman,serif;">FSHR</span></span></em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در موقعیت نوکلئوتید680 و درمان با هورمون </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">FSH</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> را نشان می&shy;دهد.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">نتیجه&shy; گیری:</span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> پلی&shy;مورفیسم </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">Asn680Ser</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> می&shy;تواند به&shy;عنوان نشان&shy;گر برای انتخاب افراد مستعد برای درمان با روش&shy;های&nbsp; </span></span><span dir="LTR"><span style="font-family:times new roman,serif;">IVF</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مورد توجه قرار گیرد. </span></span> <strong>Aim and Background<em>:</em></strong><br> Follicular stimulating hormone (FSH) is a glycoprotein and widely used for the treatment of infertility, and its action is mediated by FSH receptor (<em>FSHR</em>).There are several SNPs in FSHR gene of which determine the ovarian response to FSH. One of them is located at position 680 (Asparagine (N) to Serine (S)). Polymorphic sites give rise to three discrete variants of <em>FSHR</em>: NN, NS, and SS. We evaluated this functional polymorphism site in Iranian infertile women who undergo in vitro fertilization (either successful or failed).<br> <strong>Materials and Methods<em>:</em></strong><br> 31 incident cases and 30 controls were analyzed using PCR and direct sequencing for Asn/Ser at position 680 polymorphism.<br> <strong>Results<em>:</em></strong> In normal group Ser680/Ser680 genotype was more frequent than infertile patients. These findings failed to support the relationship between this polymorphism and infertility in evaluated Iranian women. However the total amount of FSH consumed by Ser680 in infertile patients was more than that was used by Asn680 group. A logistic regression analysis between the groups with Ser680 and Asn680 showed that Ser680 showed poor response to FSH.<br> &nbsp;<strong>Conclusions<em><u>:</u></em></strong> Our results suggested that Asn680Ser polymorphism can be used as a marker for considering FSH dosage in patients undergoing IVF, and a personalized FSH therapy may be applied based on patientś genotype at this polymorphism site in clinical setting.<br> &nbsp; گیرنده هورمون (FSHR) FSH , هورمون FSH ,پلی‌مورفیسم, IVF Follicle Stimulating Hormone, Follicle Stimulating Hormone Receptor, In-Vitro Fertilization, Single Nucleotide Polymorphisms 79 86 http://ncmbjpiau.ir/browse.php?a_code=A-10-180-2&slc_lang=fa&sid=1 Fereshteh Sarafrazi فرشته سرافرازی 10031947532846007719 10031947532846007719 No 1. Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran 1. گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی تهران Shiva Irani شیوا ایرانی 10031947532846007720 10031947532846007720 No 1. Department of Biology, School of Basic Science, Science and Research Branch, Islamic Azad University, Tehran, Iran 1. گروه زیست شناسی، دانشکده علوم پایه، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی تهران Lili Safdarian لیلی صفدریان 10031947532846007721 10031947532846007721 No 2. Department of Obstetrics and Gynecology, Shariati Hospital, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran گروه پزشکی،دانشکده علوم پزشکی،دانشگاه تهران Mohsen Ghadami محسن قدمی mghadami@tums.ac.ir 10031947532846007722 10031947532846007722 Yes 3. Department of Medical Genetics, School of medicine, Tehran University of Medical Sciences, Tehran, Iran گروه ژنتیک ،دانشکده علوم پزشکی، دانشگاه تهران