New Cellularand Molecular Biotechnology Journal
مجله تازه هاي بيوتكنولوژي سلولي و مولكولي
NCMBJ
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مطالعه و بررسی ژن مقاومت بتالاکتاماز TEM در اشریشیاکلی جدا شده از نمونههای ادراری از بیماران مراجعه کننده به درمانگاه رباط کریم
Detection of TEM beta-lactamase Gene in Escherichia Coli Isolated from Urinary Tract Infection of referred to the clinic boarding Robat Karim to the molecular method
ژنتیک
Genetics
مقاله پژوهشی
Research Article
<span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">سابقه</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">هدف</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">:</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به تازگی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">باکتر</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ی</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تولیدکننده</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بتالاکتامازهای</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">وسیع</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">الطیف</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">( ESBL)</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">که</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">توانایی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هیدرولیز</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اکثر</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آنتی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیوتیک</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بتالاکتام</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دارند،</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سراسر</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جهان</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شیوع</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">زیادی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یافت</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اند</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عنوان</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یک</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مشکل</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اساسی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">درمان</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عفونت</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">باکتریایی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطرح</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هستند</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span></span><br>
<span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">مواد و روش</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">­</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">ها</span></span></span><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">:</span></span></span></span></strong> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعه</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حاضر،</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">100</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">باکتری </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"> E. coli</span></span></span></span></em> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از نمونه­های بالینی جدا شد و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">توجه</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">استانداردهای </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">CLSI </span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و با</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">استفاده</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"> Combined Disk </span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، باکتری­های تولید کننده آنزیم بتالاکتاماز </span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شناسایی و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سپس</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">فراوانی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">TEM</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با استفاده از روش </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">PCR</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مورد بررسی قرار گرفت.</span></span></span><br>
<span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">یافته</span></span></span><strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">­ها:</span></span></span></strong><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> از 100نمونه </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">E.coli</span></span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> جدا شده ،60 نمونه مولد آنزیم بتالاکتاماز بودند واز این تعداد</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">،</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">48 نمونه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">)</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">80 درصد</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">(</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دارای ژن </span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;"> <em>TEM</em></span></span></span></span><em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بودند</span></span></span></em><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">.</span></span></span><br>
<span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">نتیجه</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">­</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">گیری</span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">:</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یافته</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اهمیت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ژن </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">TEM</span></span></span></span></em> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> را</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هیدرولیز</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داروهای بتالاکتام</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">میان</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سویه</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های </span></span></span><em><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">E.coli</span></span></span></span></em> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در شهرستان رباط کریم نشان می­دهد. شناسایی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ژن</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">وتعیین</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">پراکندگی</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آن</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">می</span></span></span><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">­</span></span></span></span><span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تواند</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روند</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تشخیص</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">درمان</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیماران</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">را</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سرعت</span></span></span> <span style="color:black;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بخشید</span></span></span><strong><span dir="LTR"><span style="color:black;"><span style="font-family:times new roman,serif;"><span style="font-size:12.0pt;">.</span></span></span></span></strong>
<strong>Aim and Background</strong><strong>:</strong> Urinary tract infections is one of the most common infectious diseases whichmany factors are involved, but bacteria such as <em>E.coli </em>is the most important agent of urinary tract infections. Antibiotic resistance as a major problem in the treatment and control of these infections is considered. The aim of this study was to determine the genes that cause resistance to betalactam family of antibiotics on <em>E.coli </em>isolated from urinary tract infections in Robat karim city.<br>
<strong>Material & Methods: </strong> In the present Cross-sectional study which was conducted over a period of<br>
6 months, 123 samples of <em>E.coli </em>were collected from Robat karim hospitals for molecular<br>
analysis of TEM genes, causing antibiotic resistance by (PCR) method.Data were<br>
analyzed using SPSS statistical test.<br>
<strong>Results: </strong>PCR showed that the gene frequency of TEM (80%) and the highest and lowest prevalent of genes were related toTEM in <em>E.coli </em>isolated from urinary tract infections respectively.<br>
<strong>Conclusion: </strong>According to the high prevalence of resistance to beta-lactam antibiotics, the current study showed that the noted geneTEM play an important role in facilitating the spread of antimicrobial resistance in this region
TEM , E.coli ,ESBL
TEM,E,coli,ESBL
85
94
http://ncmbjpiau.ir/browse.php?a_code=A-10-1280-2&slc_lang=fa&sid=1
Parvin
Mousavi
پروین
موسوی
10031947532846008144
10031947532846008144
No
Department of Biology, , Islamic Azad University, Parand Branch, Tehran, Iran
گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد پرند، پرند، ایران
Saeed
Zaker Bostan Abad
سعید
ذاکر بستان آباد
saeedzaker20@yahoo.com
10031947532846008145
10031947532846008145
Yes
Department of Biology, , Islamic Azad University, Parand Branch, Tehran, Iran
گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد پرند، پرند، ایران
Reza
Mirnejad
رضا
میرنژاد
10031947532846008146
10031947532846008146
No
Department of Microbiology, Baqiyatallah University of Medical Sciences
گروه میکروبیولوژی، دانشگاه علوم پزشکی بقیه ا....