fa بررسی ارتباط واریانت ژنتیکی rs1801275 گیرنده IL-4 در بیماران مبتلا به مالتیپل اسکلروزیس در شهر اصفهان سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">سابقه و هدف:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بیماری</span></span><span dir="LTR"><span style="font-size:10.0pt;">MS</span></span> &nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">یک بیماری التهابی و مزمن سیستم اعصاب مرکزی است</span></span><span dir="LTR"><span style="font-size:10.0pt;">.</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مطالعه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حاضر واریانت ژنتیکی </span></span><span dir="LTR"><span style="font-size:10.0pt;">rs1801275</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ژن گیرنده اینترلوکین 4 را در بیماران مبتلا به&nbsp; </span></span><span dir="LTR"><span style="font-size:10.0pt;">RRMS</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مورد بررسی قرار داده است.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">مواد و روش ها:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تعداد 100 نفر بیمار </span></span><span dir="LTR"><span style="font-size:10.0pt;">RRMS</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و 100 نفر سالم به عنوان گروه شاهد انتخاب شدند سپس استخراج </span></span><span dir="LTR"><span style="font-size:10.0pt;">DNA</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> انجام شد و پلی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">‌مورفیسم </span></span><span dir="LTR"><span style="font-size:10.0pt;">rs1801275</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> گیرنده اینترلوکین </span></span><span dir="LTR"><span style="font-size:10.0pt;">4</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با روش </span></span><span dir="LTR"><span style="font-size:10.0pt;">Real Time PCR HRM</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مورد بررسی قرار گرفت. </span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">یافته ها:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گروه شاهد 45</span></span><span dir="LTR"><span style="font-size:10.0pt;">%</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دارای ژنوتیپ طبیعی </span></span><span dir="LTR"><span style="font-size:10.0pt;">(AA)</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">،40 </span></span><span dir="LTR"><span style="font-size:10.0pt;">%</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> هتروزیگوت </span></span><span dir="LTR"><span style="font-size:10.0pt;">(AG)</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و 15</span></span><span dir="LTR"><span style="font-size:10.0pt;">%</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دارای ژنوتیپ هموزیگوت (</span></span><span dir="LTR"><span style="font-size:10.0pt;">GG</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) بودند و گروه بیمار 20 </span></span><span dir="LTR"><span style="font-size:10.0pt;">%</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دارای ژنوتیپ طبیعی</span></span><span dir="LTR"><span style="font-size:10.0pt;"> (AA)</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ، 58 </span></span><span dir="LTR"><span style="font-size:10.0pt;">%</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> هتروزیگوت(</span></span><span dir="LTR"><span style="font-size:10.0pt;"> (AG</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و 22 </span></span><span dir="LTR"><span style="font-size:10.0pt;">%</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> هموزیگوت (</span></span><span dir="LTR"><span style="font-size:10.0pt;">GG</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) بودند.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">بحث:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج پژوهش نشان داد که فراوانی آلل </span></span><span dir="LTR"><span style="font-size:10.0pt;">G</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> پلی مورفیسم </span></span><span dir="LTR"><span style="font-size:10.0pt;">rs1801275</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ژن گیرنده اینترلوکین 4 در گروه بیمار نسبت به گروه شاهد به صورت معنا داری افزایش پیدا کرده است ( 022/0=</span></span><span dir="LTR"><span style="font-size:10.0pt;">P-Value</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) .</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">نتیجه گیری:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">پلی‌مورفیسم 1801275</span></span><span dir="LTR"><span style="font-size:10.0pt;"> rs</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ژن</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گیرنده اینترلوکین 4 می تواند در افزایش احتمال ابتلا به بیماری </span></span><span dir="LTR"><span style="font-size:10.0pt;">MS</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در جمعیت مورد مطالعه نقش داشته باشد.</span></span> <strong>Aim and Background:</strong> Multiple sclerosis (MS) is an autoimmune inflammatory disease that attacks myelinated axons in the central nervous system (CNS) resulting in destroying the myelin and the axon. According to high prevalence of disease in Iran, it needs to study different aspects of disease including factors influencing the pathogenesis and the other risk factors. Various type of genetic variants including polymorphisms may could highlight the relation between genetic and disease. In this study, we explored the relationship between IL-4 receptor polymorphism rs1801275 and disease risk.<br> <strong>Material and Methods: </strong>In this study, IL-4receptor gene polymorphism was analyzed in a case-control study. All patients were selected as relapsing remitting (RR, n=100) and the control group consisted of 100 healthy (c, n= 100). High resolution melting analysis (HRMA) based on real-time PCR was performed to identify gene variation involved in disease. The results were analyzed by SPSS 20 software and also Hardy Weinberg equilibrium was tested for SNP. Also degree of heterozygosity and population analysis PIC was performed.<br> <strong>Results: </strong>The results showed samples from control group consisted of 45% wild genotype (AA), 40% heterozygote (AG) and 15% homozygote (GG) for polymorphism rs1801275 while there was in patient group 20% wild genotype (AA), 58% heterozygote (AG) and 22% homozygote (GG).<br> <strong>Conclusion:</strong> These results suggest that there was significant difference in allele count between control and patient groups. The allele frequency of IL-4 receptor polymorphism rs1801275was found significantly higher in patients (P-Value = 0.022). IL4R polymorphisms may have a disease-promoting role in this population.<br> &nbsp; مالتیپل‌اسکلروزیس, واریانت ژنتیکی, rs1801275, گیرنده اینترلوکین 4. Multiple Sclerosis, Genetic Variants, rs1801275, Interleukin 4 receptor. 93 102 http://ncmbjpiau.ir/browse.php?a_code=A-10-1329-2&slc_lang=fa&sid=1 Maryam Azadi مریم آزادی 100319475328460011026 100319475328460011026 No .Department of Biology, Faculty of Basic Sciences, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran گروه زیست شناسی، دانشکده علوم پایه، واحد شهرکرد، دانشگاه آزاد اسلامی، شهرکرد، ایران Fereshteh alsahebfosoul فرشته آل صاحب فصول alsahebfosoul@med.mui.ac.ir 100319475328460011027 100319475328460011027 No Department of Immunology, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan , Iran گروه ایمنی شناسی دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان Rasoul Salehi رسول صالحی 100319475328460011028 100319475328460011028 Yes Department of Molecular Biology and Genetics, Faculty of Medicine, Isfahan University of Medical Sciences, Isfahan Iran. گروه بیولوژی ملکولی و ژنتیک دانشکده پزشکی، دانشگاه علوم پزشکی اصفهان Elaheh Tajbakhsh الهه تاجبخش 100319475328460011029 100319475328460011029 No Department of Biology, Azad University, Shahrekord Branch , Shahrekord, Iran گروه زیست شناسی دانشگاه آزاد واحد شهرکرد Masoud Etemadifar مسعود اعتمادی فر 100319475328460011030 100319475328460011030 No Research Center for MS and Neuroimmunology in Isfahan, Isfahan , Iran مرکز تحقیقات MS و نوروایمونولوژی اصفهان