fa نانو حاملی بر گرفته از آرکی باکترها تحت عنوان آرکئوزوم و بررسی بار گذاری داروی سیس پلاتین بر روی آن میکروبیولوژی Microbiology مقاله پژوهشی Research Article <p align="justify"> <strong>سابقه </strong><strong></strong><strong>و </strong><strong></strong><strong>هدف </strong><strong>: </strong>آرکئوزرم به عنوان یکی از حامل های لیپیدی جدید برای دارورسانی هدفمند، جهت درمان انواع سرطان شناخته شده است . برای حذف اثرات جانبی دارو بر سایر سلول ها و رسیدن دوز بالاتری از دارو به سلول هدف، داروی ضد سرطان سیس پلاتین در آرکئوزرم کپسوله می شود. </p><p align="justify"> <strong>مواد </strong><strong></strong><strong>و </strong><strong></strong><strong>روش </strong><strong></strong><strong>ها </strong>: آرکئوزرم از آرکی باکتر های متانوژن استخراج شد و پس از انحلال در بافر فسفات، به منظور بارگذاری سیس پلاتین بر آرکئوزرم ساخته شده و تغییر اندازه ذرات به ابعاد نانو، محلول در سونیکاتور حمام دار سونیکه شد . </p><p align="justify"><strong>یافته </strong><strong></strong><strong>ها </strong><strong>: </strong>موازنه جرم با استفاده از دانسیته و جرم مولکولی مواد تشکیل دهنده محلول سونیکه شده محاسبه شد . سپس، میزان درصد بارگذاری دارو بر روی نانوآرکئوزرم حاصل گردید و در نهایت با استفاده از دستگاه زتاسایزر اندازه ذرات در ابعاد نانو تایید شد . </p><p align="justify"> <strong>نتیجه </strong><strong></strong><strong>گیری </strong><strong>: </strong>هدف از پژوهش حاضر، تولید نانوآرکئوزرم حامل داروی سیس پلاتین بود که به منظور دارورسانی هدفمند، جهت درمان انواع سرطان به کار گرفته می شود که با درصد بارگذاری مناسب به این مهم دست یافتیم . </p><p align="justify"> <strong> </strong></p> <p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><span class="CharacterStyle1"><font face="Times New Roman"><font size="2"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 14pt">Aim<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>and<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>Background<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>.</span></span></b><span dir="rtl" lang="AR-YE"> </span>Archaeosome<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>is<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>known<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>as<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>a<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>new<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>lipid<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>carrier<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>for<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>targeted<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>drug<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>delivery<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>in<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>cancer<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>therapy<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>. </span>The<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>anti<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>cancer<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>drug<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span> , </span>Cisplatin<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span> , </span>is<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>encapsulated<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>in<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>archaeosome<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>to<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>reduce<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>the<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>side<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>effects<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>of<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>drug<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>on<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>other<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>cells<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>and<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>offer<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>more<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>dosage<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>of<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>drug<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>on<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>target<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>cell</font></font></span></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><span class="CharacterStyle1"></span><span class="CharacterStyle1"><font face="Times New Roman"><font size="2"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 14pt">Material<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>and<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>Method<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>.</span></span></b><span dir="rtl" lang="AR-YE"> </span>Archaeosome<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>was<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>extracted<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>from<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>methanogens<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>and<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>after<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>being<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>dissolved<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>in<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>phosphate<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>buffer<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>, </span>the<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>solution<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>was<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>sonicated<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>in<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>bath<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>sonicator<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>for<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>loading<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>the<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>cisplatin<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>on<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>extracted<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>archaeosome<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>and<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>resize<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>to<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>nano<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>scale<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>.</span></font></font></span><p></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><span class="CharacterStyle1"><font face="Times New Roman"><font size="2"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 14pt">Results<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>.</span></span></b><span dir="rtl" lang="AR-YE"> </span>Mass<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>balance<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>was<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>obtained<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>using<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>density<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>and<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>molecular<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>weight<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>of<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>sonicated<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>solution<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>elements<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>, </span>and<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>therefore<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>, </span>the<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>loading<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>percentage<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>of<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>drug<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>on<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>nano<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>archaeosome<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>was<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>obtained<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>. </span>The<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>particles<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>size<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>in<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>nano<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>scale<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>was<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>confirmed<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>with<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>zeta<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>sizer<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>. </span></font></font></span><p></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><span class="CharacterStyle1"><font face="Times New Roman"><font size="2"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 14pt">Conclusion<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>.</span></span></b><span dir="rtl" lang="AR-YE"> </span>The<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>main<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>purpose<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>of<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>this<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>research<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>was<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>to<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>load<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>cisplatin<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>on<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>archaeosome<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>for<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>targeted<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>drug<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>delivery<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>against<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>cancer<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>. </span>We<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>obtained<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>very<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>suitable<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>loading<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>percentage<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>that<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>allowed<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>us<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>to<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>succeed<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>in<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>our<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span> </span>aim<span dir="rtl"></span><span dir="rtl" lang="AR-YE"><span dir="rtl"></span>.</span></font></font></span><p></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><span class="CharacterStyle1"><span dir="rtl" lang="AR-YE"></span></span></p></p><p></p></p><p></p></p><p></p> آرکئوزرم، سیس پلاتین، دارورسانی هدفمند archaeosome, cisplatin , targeted drug delivery , loading 9 13 http://ncmbjpiau.ir/browse.php?a_code=A-10-61-3&slc_lang=fa&sid=1 Alireza Bozorgnejad علیرضا بزرگ نژاد abnejad62@gmail.com 1003194753284600801 1003194753284600801 Yes science and research branch, Islamic Azad University دانشکده مهندسی ، دانشگاه آزاد اسلامی واحد علوم و تحقیقات Fatemeh Baghi فاطمه باغی 1003194753284600802 1003194753284600802 No science and research branch, Islamic Azad University دانشکده مهندسی ، دانشگاه آزاد اسلامی واحد علوم و تحقیقات Azim Akbarzadeh عظیم اکبرزاده 1003194753284600803 1003194753284600803 No Department of pilot biotechnology , Pasteur Institute of Iran , Tehran , Iran پایلوت بیوتکنولوژی ، انستیتو پاستور ایران Mehdi Mehdi Arjmand مهدی ارجمند 1003194753284600804 1003194753284600804 No chemical engineering , Department of chemical engineering, South Tehran branch, Islamic Azad University, Tehran, Iran گروه مهندسی شیمی ، دانشگاه آزاد اسلامی واحد تهران جنوب ، تهران ، ایران