fa بررسی بیان ژن YKL39 هدف و miR آن در بافت بیماران گلیوبلاستومامولتی فرم ژنتیک Genetics مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف</span></span></b><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> یکی از بدخیم ترین و رایج ترین تومورهای مغزی گلیوبلاستومامولتی&shy;فرم (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">GBM</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) است.</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بقای بیمار نسبتاً ضعیف است و</span></span>&nbsp;<span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با توجه به دشوار بودن تشخیص زود هنگام اکثر بیماران طی یک سال بعد از تشخیص می&shy;میرند</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> هدف از این مطالعه بررسی </span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">بیان</span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="background:white"><span new="" roman="" style="font-family:" times="">YKL39</span></span></span></i><i> </i><i>&nbsp;</i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به عنوان بیومارکر احتمالی در گلیو</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بلاستومامولتی فرم و بررسی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">miR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> این ژن می باشد. </span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مواد و روش ها:</span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در این مطالعه 25 نمونه بافت مبتلا به گلیوبلاستومامولتی فرم قبل از درمان و 25 نمونه بافت سالم حاشیه تومور گلیوبلاستومامولتی فرم</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">عنوان</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نمونه</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کنترل</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">جمع آوری</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شد</span></span> <span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">استخراج</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">RNA</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">سنتز</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">cDNA</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;صورت</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">گرفت. بیان</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن</span></span> <i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">YKL39</span></span></i><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با استفاده از روش </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Real-time PCR</span></span> &nbsp;<span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شد و از </span></span><span lang="AR-SA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">ژن</span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">ACTB</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;به عنوان کنترل داخلی استفاده شد.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تجزیه</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و تحلیل</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آماری</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">داده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با استفاده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نرم</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">افزار</span></span> <span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">GraphPad Prism</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نسخه</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">8</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;انجام شد</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> برای بررسی ارزش بیومارکری ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">YKL39</span></span></i><i> </i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از منحنی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Roc</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استفاده شد. </span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">یافته ها:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیان بالای </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">YKL39</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span>^{<span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">****</span></span>}<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><0001/0) در نمونه های بافت گلیوبلاستوما مشاهده شد.<i> بیان</i> نشان داد ژن </span></span>&nbsp;<i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">YKL39</span></span></i><span lang="FA" style="font-size:10.0pt"><span b="" nazanin="" style="font-family:">با</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سن بیماران با </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">2971/0</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">=</span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span>_{<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">value</span></span>}<span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;و جنسیت</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">8988/</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">0= </span></span>&nbsp;<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">P</span></span> _{<span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">value</span></span>} <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از لحاظ آماری معنادار نمی باشد. </span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه گیری:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> افزایش بیان ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">YKL39</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در نمونه های بافت گلیوبلاستوما در مقایسه با بافت سالم حاشیه تومور قابل توجه است. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">منحنی </span></span><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">Roc</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نشان داد که ژن </span></span><i><span dir="LTR" style="font-size:10.0pt"><span new="" roman="" style="font-family:" times="">YKL39</span></span></i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نمی تواند دارای ارزش بیومارکری باشد<i>.</i></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Aim and Background:</span></b><span new="" roman="" style="font-family:" times=""> One of the most malignant and common brain tumors is glioblastoma multiforme (GBM). Patient survival is relatively poor, and due to the difficulty of early diagnosis, most patients die within one year of diagnosis. The aim of this study was to investigate the expression of <i>YKL39</i> as a possible biomarker in glioblastoma multiforme and to evaluate the miR of this gene.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span dir="RTL" style="font-family:"Times New Roman","serif"" lang="AR-SA"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Materials and Methods:</span></b><span new="" roman="" style="font-family:" times=""> In this study, 25 tissue samples with glioblastoma multiforme before treatment and 25 samples of healthy tissue with glioblastoma multiforme tumor were collected as a control sample. RNA extraction and cDNA synthesis were performed. <i>YKL39</i> gene expression was assessed using real-time PCR and ACTB gene was used as internal control. Statistical analysis of data was performed using GraphPad Prism software version 8. The Roc curve was used to evaluate the biomarker value of <i>YKL39</i> gene.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span dir="RTL" style="font-family:"Times New Roman","serif"" lang="AR-SA"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Results:</span></b><span new="" roman="" style="font-family:" times=""> High expression of <i>YKL39</i> (P **** <0.0001) was observed in glioblastoma tissue samples. Expression showed that <i>YKL39</i> gene was not statistically significant with the age of patients with P value = 0.2971 and gender with P value = 0.888.</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span dir="RTL" style="font-family:"Times New Roman","serif"" lang="AR-SA"></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span new="" roman="" style="font-family:" times="">Conclusion:</span></b><span new="" roman="" style="font-family:" times=""> The increase in <i>YKL39</i> gene expression in glioblastoma tissue samples is significant compared to healthy tumor margin tissue. The Roc curve showed that the <i>YKL39</i> gene could not have biomarker value.</span></span></span></span><br> &nbsp; YKL39, گلیوبلاستوما مولتی فرم,Real-Time PCR , بیومارکر., Iau Science. YKL39, Glioblastoma multiforme, Real-Time PCR, Biomarker, Iau Science. 89 99 http://ncmbjpiau.ir/browse.php?a_code=A-10-1740-2&slc_lang=fa&sid=1 Atiyeh Tavakoli عطیه توکلی 100319475328460012859 100319475328460012859 No 1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University Damghan Branch, Damghan, Iran گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران Hamidreza Kheiri حمیدرضا خیری 100319475328460012860 100319475328460012860 No 2. Department of Pharmaceutical Nanotechnology, School of Pharmacy, Zanjan Universityof Medical Sciences, Zanjan, Iran گروه زیست‌ فناوری دارویی، دانشگاه علوم پزشکی، زنجان، ایران Vajiheh Zarrinpour وجیهه زرین پور zarrinpour @damghaniau.ac.ir. 100319475328460012861 100319475328460012861 No 1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University Damghan Branch, Damghan, Iran گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران Mohammadmahdi Forghanifard محمد مهدی فرقانی فرد 100319475328460012862 100319475328460012862 Yes 1. Department of Biology, Faculty of Basic Sciences, Islamic Azad University Damghan Branch, Damghan, Iran گروه زیست‌شناسی، واحد دامغان، دانشگاه آزاد اسلامی، دامغان، ایران