fa تاثیر نانوذرات نقره بر قدرت زنده مانی سلول‌های نرمال پوست (MHFB-1) سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">چکیده</span></span></b></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف: </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نانو&shy;ذرات</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نقره(</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">AgNPs</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به واسطه اندازه کوانتومی خود خواص فیزیکی و شیمیایی منحصر به فردی دارند که منجر به طیف گسترده ای از کاربردهای زیست پزشکی قابل توجه می گردد. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با این وجود </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اطلاعات</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اندکی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">رابطه</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تاثیرنانوذرات</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سلول&shy;های سالم وجود</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دارد. این</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تحقیق با هدف بررسی تاثیر</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نانوذرات&shy;نقره</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر درصد زنده مانی رده سلول نرمال پوست </span></span><i><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">MHFB-1</span></span></i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام&shy;گرفت.</span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مواد و روش</span></span></b><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">&shy;</span></span></b><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">ها:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مطالعه،</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در طی24&shy;، 48 و 72&shy; ساعت،</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">غلظت&shy;های مختلف نانو&shy;ذرات&shy;نقره( 56/1، 125/3، 25/6، 5/12&shy;، 25&shy;، 50&shy; </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">100 میکروگرم بر میلی&shy;لیتر)</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر رده سلولی</span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">MHFB-1 </span></span></i><i>&nbsp;</i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> روش</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">MTT</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;بررسی شد. </span></span><span b="" nazanin="" style="font-family:">داده&shy;های حاصل از تست مذکور با استفاده از نرم افزار </span>SPSS <span b="" nazanin="" style="font-family:">&nbsp;،</span><span b="" nazanin="" style="font-family:">آزمون</span> <span b="" nazanin="" style="font-family:">.واریانس یک طرفه، &nbsp;تست توکی و سطح معنی داری</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> 05/0</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">></span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">P</span></span><span b="" nazanin="" style="font-family:"> مورد تجزیه و تحلیل آماری قرار گرفت. </span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">نتایج:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تیمار سلول&shy;های نرمال</span></span><i><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">MHFB-1</span></span></i><i> </i><i>&nbsp;</i><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با غلظت&shy;های مختلف نانو&shy;ذرات &shy;نقره بعداز 2</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">4&shy;، 48 و 72&shy; ساعت</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با روش</span></span><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">MTT</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، کاهش معنی&shy;دار</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بقاء</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سلول&shy;ها&nbsp; را در غلظت&shy;های &nbsp;25، 50 </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">100&shy; میکروگرم بر میلی&shy;لیتر را نشان داد.</span></span><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="AR-SA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">نتیجه گیری:</span></span></b><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> نتایج</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تاثیرمهاری وابسته به دوز نانو&shy;ذرات&shy;</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نقره &nbsp;بر</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> رده</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سلول&shy;</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نرمال پوست ( </span></span><i><span dir="LTR" lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">MHFB-1</span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">)را نشان داد.</span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:12pt"><span style="background:white"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="letter-spacing:-.1pt">Aim and Background</span></b><b>: </b>Silver nanoparticles (AgNPs) have been considered by researchers as a very strong inhibitor due to their biological properties and have many applications in prevention and treatment. However, there is little information about the effect of nanoparticles on healthy cells.The aim of this study was to investigate the effect of silver nanoparticles on the viability percentage of normal skin cell line MHFB-1.</span></span></span></span><br> <span style="font-size:12pt"><span style="background:white"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b>Material and methods:</b>In this study, during 24, 48 and 72 hours, different concentrations of silver nanoparticles (1.56, 3.125, 6.25, 12.5, 25, 50 and 100 &mu;g / ml on MHFB-1 cell line was evaluated by MTT method.Data obtained from the test were statistically analysed using SPSS software, one-way ANOVA, Tukey test and significance level of P <0.05.</span></span></span></span><br> <br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Results:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> Treatment of normal <i>MHFB-1</i> cells with different concentrations of silver nanoparticles after 24, 48 and 72 hours by &nbsp;MTT method showed a significant reduction &nbsp;in </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">the viability of cells </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">at &nbsp;concentrations of 25, 50 and 100</span></span><span lang="EN-GB" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> &mu;g /ml</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="text-autospace:none"><span style="line-height:115%"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Conclusion</span></span></span></b><b><span dir="RTL" lang="AR-SA" style="font-size:12.0pt"><span style="line-height:115%"><span b="" nazanin="" style="font-family:">:</span></span></span></b> <span lang="EN-GB" style="font-size:12.0pt"><span style="line-height:115%"><span new="" roman="" style="font-family:" times="">Conclusion: The results of this study showed a dose-dependent inhibitory effect of silver nanoparticles on normal skin cell line (MHFB-1).</span></span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span style="line-height:115%"><span style="font-family:"Times New Roman","serif""></span></span></span></span></span></span></span></span><br> &nbsp; نانو‌ذرات ‌نقره, زنده مانی, روش رنگ سنجی سمیت سلولی(MTT), رده سلول نرمال پوست, Iau Science. Silver nanoparticles,Viability, MTT assay ,Normal skin cells line., Iau Science. 45 54 http://ncmbjpiau.ir/browse.php?a_code=A-10-1664-3&slc_lang=fa&sid=1 Mohamad Hemati محمد همتی 100319475328460012890 100319475328460012890 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران. Zahra Keshtmand زهرا کشتمند zkeshtmand2001@gmail.com 100319475328460012891 100319475328460012891 No Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran گروه زیست شناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران. Katayoun Borhani کتایون برهانی 100319475328460012892 100319475328460012892 Yes Department of Environment and Food industry Engineering, Central Tehran Branch, Islamic Azad University, Tehran, Iran. گروه مهندسی محیط زیست و صنایع غذایی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران.