fa آنالیز مقایسه‌ای ترانسکریپتوم و شناسایی ژن‌های کلیدی مقاومت به کم‌آبی در ریشه گندم (Triticum aestivum L.) تلقیح‌شده با میکوریزا آربوسکولار (Funneliformis mosseae) گیاه شناسی Botany مقاله پژوهشی Research Article <div class="WordSection1" dir="RTL"><span style="page:WordSection1"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">چکیده</span></span></b></span></span></span></span></span><br> <span style="font-size:10pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span antiqua="" book="" style="font-family:"><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">قارچ‌های میکوریزا آربوسکولار</span></span> <span dir="LTR" style="font-size:11.0pt"><span new="" roman="" style="font-family:" times="">(AMF)</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سازگاری و </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تحمل به تنش را در گیاه همزیست بهبود می‌بخشند</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در شرایط تنش کم‌آبی، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">همزیستی میکوریزایی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با تغییر </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> الگوی بیان </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن‌های گیاه میزبان همراه است</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی پروفایل بیانی القا شده </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">توسط</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">میکوریزا</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در گیاه میزبان،</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">برای درک </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مکانیزم </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مولکولی افزایش </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مقاومت </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کم‌آبی ضروری</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> است.</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این پژوهش با هدف مطالعه ترانسکریپتومی ریشه گندم، به</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">منظور </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شناسایی </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مکانیزم‌های مولکولی‌</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تحمل</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به کم‌آبی</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> واسطه میکوریزا </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">انجام شد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">مواد و روش &shy;ها:</span></span></b> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در تحقیق حاضر</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> الگوی ترانسکریپتوم ریشه گندم</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نان </span></span><i>Triticum aestivum</i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> (رقم </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">چمران</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">) </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تلقیح شده </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با قارچ میکوریزا آربوسکولار </span></span><i>Funneliformis mosseae</i><i> </i><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در 4 تیمار: گیاهان تلقیح شده با میکوریزا در شرایط </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کم‌آبی</span></span> (AMF+/WD)<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> گیاهان تلقیح </span></span>&shy;<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شده با میکوریزا در شرایط آبیاری نرمال </span></span>(AMF+/Ctrl)<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، گیاهان تلقیح نشده تحت شرایط </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کم‌آبی</span></span> (AMF-/WD)<span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و گیاهان تلقیح‌ نشده در شرایط آبیاری نرمال </span></span>(AMF-/Ctrl) <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بررسی</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> شد. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">پـس از بررسی کیفی و کمی و ساخت کتابخانه </span></span>cDNA <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تعیین توالی</span></span> RNA<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> انجام شد و</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> داده‌های حاصل از </span></span>RNA-Seq<span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> توسط نرم‌افزارهای مربوطه</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">آنالیز گردید.</span></span><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Time new roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">یافته &shy;ها</span></span></b><b><span lang="FA" style="font-size:18.0pt"><span b="" nazanin="" style="font-family:">:</span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در مجموع 235 </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن با بیان افتراقی</span></span> (DEGs) <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از 4 مقایسه شناسایی شد</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر‌اساس تجزیه و تحلیل نمودار </span></span>Venn<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، 70 ژن شناسایی شد که</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به&shy;طور انحصاری </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در شرایط تنش کم‌آبی </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">توسط </span></span>AMF <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">القاء می‌شوند. </span></span><b><span dir="LTR" style="font-size:18.0pt"></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:18.0pt"><span b="" nazanin="" style="font-family:">بحث:</span></span></b><b> </b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تجزیه و تحلیل </span></span><span dir="LTR" new="" roman="" style="font-family:" time="">GO</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" new="" roman="" style="font-family:" time="">KEGG</span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نشان داد ژن‌های دارای بیان افتراقی </span></span>(DEGs) <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در مسیرهای مختلف و متعددی مشارکت دارند</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">. </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیشترین</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تعداد </span></span>(DEGs)<span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> در </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مسیرهای بیو</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سنتز و </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">شکل‌گیری </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">دیواره سلولی، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تنظیم ردوکس سلولی و </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تنظیم اسمزی نظیر بیوسنتز پرولین</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">، شناسایی شدند. </span></span><b><span lang="FA" style="font-size:18.0pt"><span style="font-family:"B Nazanin""></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">نتیجه</span></span></b><span dir="LTR" style="font-size:16.0pt"><span new="" roman="" style="font-family:" time="">&shy;</span></span><b><span lang="FA" style="font-size:16.0pt"><span b="" nazanin="" style="font-family:">گیری:</span></span></b> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">این نتایج نشان می‌دهد میکوریزا با ا</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">عمال </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">تنظیمات دقیق، </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بیان </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن‌های کلیدی پاسخ‌ به استرس در ریشه گیاه را تغییر می‌دهد </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">و</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">بر ظرفیت سازگاری گیاه گندم </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در طول کمبود آب </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">می‌افزاید<span style="display:none">می‌فزمد</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">.</span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">از </span></span><span b="" nazanin="" style="font-family:">این </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ژن‌ها می‌توان </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در برنامه‌های</span></span> <span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">اصلاحی و مهندسی ژنتیک گندم </span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">جهت بهبود تحمل به </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">کم‌آبی </span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">استفاده کرد.</span></span> <span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="FA" style="font-size:12.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span><br> &nbsp;</div> <div aria-label="شکستن صفحه" class="cke_pagebreak" contenteditable="false" data-cke-display-name="pagebreak" data-cke-pagebreak="1" style="page-break-after:always" title="شکستن صفحه"></div> <div align="right" dir="RTL" style="text-align:left"> <hr align="right" noshade="noshade" size="3" style="color:#404040" width="97%" ></div> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:16.0pt"><span style="line-height:107%"><span style="font-family:"B Nazanin""></span></span></span></b></span></span></span></span></span><br> <br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><span lang="RU" dir="LTR" style="font-size:12.0pt"></span></span></span></span></span></span> تنش کم‌آبی (WD), ترانسکریپتوم, گندم, همزیستی میکوریزی, پرولین 95 114 http://ncmbjpiau.ir/browse.php?a_code=A-10-1834-1&slc_lang=fa&sid=1 الهه دوخه 100319475328460014145 100319475328460014145 No Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران علیرضا ایرانبخش iranbakhsh@iau.ac.ir 100319475328460014146 100319475328460014146 Yes Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran. گروه زیست شناسی، واحد علوم و تحقیقات، دانشگاه آزاد اسلامی، تهران، ایران ایرج مهرگان iraj@daad-alumni.de 100319475328460014147 100319475328460014147 No رحیم احمدوند r.ahmadvand@areeo.ac.ir 100319475328460014148 100319475328460014148 No مصطفی عبادی mtf.ebadi@gmail.com 100319475328460014149 100319475328460014149 No