fa تعیین دوز موثر کادمیوم بر میزان زیستایی و بیان ژن کانکسین 43 در رده سلولی RAW264-7 فیزیولوژی Physiology مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">سابقه و هدف: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">فلزات سمی مانند کادمیوم و استرس اکسیداتیو ناشی از آن</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">&shy;</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">ها می&shy;تواند موجب پیشرفت بسیاری از بیماری&shy;ها همچون آترواسکلروزیس شوند. آترواسکلروزیس یک پدیده&shy;ی پیش رونده التهابی مزمن است که با تجمع چربی&shy;ها در محل انشعاب عروق منجر به بروز بیماری&shy;های ایسکمی دهنده می&shy;شود. اختلال در عملکرد ژن&shy;های بسیاری همچون کانکسین&shy;ها که در هموستاز چربی&shy;ها نقش دارند می&shy;تواند موجب بروز آترواسکلروزیس شود. مطالعه حاضر به بررسی اثر کادمیوم بر میزان بیان ژن کانکسین 43 در رده سلولی </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">RAW264-7</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> می&shy;پردازد .</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">مواد و روش ها: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">در این پژوهش<b> </b>رده سلولی </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">RAW264-7</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> کشت داده و تحت تاثیر دوزهای مختلف کلرید کادمیوم قرار داده شد. </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IC50</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> با استفاده از تست </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">MTT</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> تعیین، </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">RNA</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> استخراج و میزان بیان ژن کانکسین 43 به روش </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Real-time PCR </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;&nbsp;ارزیابی شد. نتایج با استفاده از نرم&shy;افزار آماری </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">&nbsp;SPSS</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">با روش آماری </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">one way ANOVA </span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;آنالیز گردید. </span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">یافته &shy;ها: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتایج نشان می&shy;دهد که بیان ژن کانکسین 43 در سلول&shy;های آلوده شده توسط دوز 3/6 میکرومولار کادمیوم در زمان کوتاه نسبت به گروه کنترل افزایش معنی&shy;داری نشان می&shy;دهد. همچنین نتایج </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">IC₅₀</span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> بیانگر کاهش زیستایی سلول&shy;های در این غلظت&shy; می&shy;باشد.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:301.3pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">نتیجه&shy; گیری: </span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">به نظر می&shy;رسد کادمیوم در غلظت&shy;های متوسط و در زمان کوتاهی می&shy;تواند با افزایش بیان ژن کانکسین 43 در رده سلولی</span></span><span dir="LTR" new="" roman="" style="font-family:" times="">RAW264-7 </span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:">&nbsp;اثرات سایتوتوکسیک بر گیرنده&shy;های </span></span><span dir="LTR" new="" roman="" style="font-family:" times="">Scavenger</span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"> سلول&shy;های ماکروفاژی گذاشته و منجر به پیشرفت آتروسکلروزیس شود.</span></span></span></span></span></span></span></span><br> &nbsp; <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Aim and Background:</span></span></b> <span new="" roman="" style="font-family:" times="">Toxic</span><span new="" roman="" style="font-family:" times=""> metals such as cadmium and the oxidative stress caused by them can develop many Disease such as atherosclerosis. Atherosclerosis is a progressive chronic inflammatory disease that leads to the coronary ischemic with the accumulation of fats at the branching point of the vessels. Dysfunction of many genes, such as connexin, which play a role in lipid homeostasis, can cause atherosclerosis. The present study investigates the effect of cadmium on the expression level of connexin 43 gene in RAW264-7 cell line. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="EN" style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Material and methods:</span></span></b><span new="" roman="" style="font-family:" times=""> In this study, RAW264-7 cell line was cultured and affected by different doses of cadmium chloride. IC₅₀ was determined using MTT test, RNA was extracted and connexin 43 gene expression was evaluated by Real Time PCR method. The results were analyzed using SPSS software with </span><span new="" roman="" style="font-family:" times="">o</span><span new="" roman="" style="font-family:" times="">neway ANOVA statistical method.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif">&nbsp;</span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Result:</span></span></b><span new="" roman="" style="font-family:" times=""> Our findings showed that the expression of connexin 43 gene in polluted cells by 6.3 &mu;M cadmium had a significant increase compared with control group, especially in a short time. Also, the IC₅₀ results indicate a decrease in cell viability in this concentration. </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="EN" style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span new="" roman="" style="font-family:" times=""> It seems that cadmium in moderate concentrations and in a short time can exert cytotoxic effects on the scavenger receptors of macrophage cells by increasing the expression of connexin 43 gene in RAW264-7 cell line and lead to the development of atherosclerosis.</span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> <br> <span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="EN" style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> &nbsp; کادمیوم , کانکسین 43 , رده سلولیRAW264-7  , آتروسکلروزیس, Iau Science. cadmium, connexin 43, cell line RAW264-7, atherosclerosis, Iau Science. 43 52 http://ncmbjpiau.ir/browse.php?a_code=A-10-121-1&slc_lang=fa&sid=1 Vahid Naseh وحید ناصح Vahid55vet@yahoo.com 100319475328460016295 100319475328460016295 Yes Department of Biology, Parand Branch, Islamic Azad university, Tehran, Iran. گروه زیست شناسی، واحد پرند، دانشگاه آزاد اسلامی. تهران. ایران Masoud Salehypour مسعود صالحی پور 100319475328460016296 100319475328460016296 No Ameneh Moghadas آمنه مقدسی 100319475328460016297 100319475328460016297 No Sahar Farzaneh سحر فرزانه 100319475328460016298 100319475328460016298 No