fa نقش نانوذرات نقره زیستی سنتز شده با عصاره Serenoa repens بر شاخص‌های فیزیولوژی گیاهی و پایداری غشایی در یک گیاه دارویی تحت تنش خشکی گیاه شناسی Botany مقاله پژوهشی Research Article <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">چکیده</span></span></span></b><span dir="LTR" style="font-size:16.0pt"><span style="line-height:107%"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">مقدمه</span></span></span></b><b><span dir="LTR" style="font-size:16.0pt"><span style="line-height:107%">:</span></span></b><br> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">تنش خشکی یکی از مهم‌ترین عوامل محدودکننده رشد و تولید ثانویه در گیاهان دارویی است که با ایجاد استرس اکسیداتیو، به آسیب ساختارهای سلولی و کاهش فتوسنتز منجر می‌شود. استفاده از نانوذرات زیستی حاصل از عصاره‌های گیاهی، روشی نوین و پایدار برای تحریک سیستم دفاعی گیاهان در برابر تنش‌های محیطی محسوب می‌شود. در این مطالعه، برای نخستین‌بار از نانوذرات نقره زیستی سنتز شده با عصاره </span></span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">Serenoa repens</span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">جهت القای تحمل به خشکی در یک گیاه دارویی استفاده شد</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">مواد و روش‌ها</span></span></span></b><b><span dir="LTR" style="font-size:16.0pt"><span style="line-height:107%">:</span></span></b><br> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نانوذرات نقره از عصاره آبی </span></span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">S. repens</span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">سنتز و با روش‌های</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> UV-Vis (</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">پیک جذب </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۴۳۵</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> نانومتر</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">)</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">FTIR </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">و</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> SEM (</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">میانگین اندازه </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۳۲</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٫</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۶</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> نانومتر) شناسایی شدند. گیاهان تحت تیمار نانوذره در سه غلظت (</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۰</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۲۵</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> و </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۵۰</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> میلی‌گرم بر لیتر) و تنش خشکی (</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۴۰</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٪</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> ظرفیت زراعی) به‌مدت </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۱۵</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> روز قرار گرفتند. شاخص‌های</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> RWC</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، کلروفیل کل، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">MSI</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، فعالیت آنزیم‌های آنتی‌اکسیدانی</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> (SOD</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">CAT</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">POD) </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">و سطح</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> MDA </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">ارزیابی شدند</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نتایج</span></span></span></b><b><span dir="LTR" style="font-size:16.0pt"><span style="line-height:107%">:</span></span></b><br> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">غلظت </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۵۰</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> میلی‌گرم بر لیتر نانوذره سبب افزایش معنی‌دار</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> RWC (</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۲۸</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٫</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۳</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٪</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">)</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، کلروفیل کل (</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۲۱</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٫</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۷</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٪</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">و</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> MSI (</span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۳۴</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٫</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۱</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٪</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">) </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">شد. فعالیت</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> SOD</span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، </span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">CAT </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">و</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> POD </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">به‌ترتیب </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۴۷</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٫</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۵</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٪</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">، </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۳۹</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٫</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۲</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٪</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> و </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۴۲</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٫</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۶</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٪</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> افزایش و میزان</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%"> MDA </span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">به میزان </span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۳۱</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٫</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">۸</span></span></span><span lang="FA" style="font-size:12.0pt"><span style="line-height:107%"><span new="" roman="" style="font-family:" times="">٪</span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:"> کاهش یافت</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">.</span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="line-height:107%"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:16.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">بحث و نتیجه‌گیری</span></span></span></b><b><span dir="LTR" style="font-size:16.0pt"><span style="line-height:107%">:</span></span></b><br> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">نانوذرات نقره زیستی </span></span></span><i><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">S. repens</span></span></i> <span lang="AR-SA" style="font-size:12.0pt"><span style="line-height:107%"><span b="" nazanin="" style="font-family:">با بهبود وضعیت آبی، پایداری غشاء و تحریک سیستم آنتی‌اکسیدانی، تحمل به خشکی را به‌طور مؤثری افزایش دادند. این ترکیبات می‌توانند به‌عنوان ابزار زیست‌سازگار در کشاورزی پایدار و افزایش تاب‌آوری گیاهان دارویی در برابر تنش‌های اقلیمی مورد استفاده قرار گیرند</span></span></span><span dir="LTR" style="font-size:12.0pt"><span style="line-height:107%">.</span></span></span></span></span></span></span><br> &nbsp; واژگان کلیدی: نانوذرات نقره زیستی · Serenoa repens · تنش خشکی · آنزیم‌های آنتی‌اکسیدانی 0 0 http://ncmbjpiau.ir/browse.php?a_code=A-10-2084-4&slc_lang=fa&sid=1 بهزاد امرایی b.amraei@pnu.ac.ir 1741369983 100319475328460015929 Yes دانشگاه پیام نور تهران