other تأثیر نانوذرات اکسید آهن عامل‌دار شده با هیالورونیک اسید بر بیان‌ژن CD44 و رشد ردۀ سلولیِ 1-Aspc سرطان لوزالمعده سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <span style="font-size:14pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سابقه و هدف: </span></span></span></b><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سرطان لوزالمعده رتبۀ </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سوم</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> مرگ‌ومیرهای ناشی از سرطان است. هیالورونیک اسید به‌دلیل جذب اختصاصی و نداشتن عوارض جانبی در ‌درمان می‌تواند مورد استفاده قرار گیرد. هدف از این پژوهش بررسی تأثیر</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> نانوذرات اکسید آهن و هیالورونیک</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بر</span></span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">تغییرات بیان‌ژن </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="color:black">CD44</span></span></i><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> در سلول‌های سرطانی</span></span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بود.</span></span></span></span></span></span></span></span><br> <span style="font-size:14pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">مواد و روش‌ها: </span></span></span></b><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">در </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">این </span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">پژوهش </span></span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نانوذرات اکسید آهن به‌روش هم‌رسوبی، سنتز و با هیالورونیک اسید سنتز عامل‌دار شدند. از تست‌های تأییدی میکروسکوپ الکترونی روبشی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">SEM</span></span><span lang="AR-SA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">)</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">، طیف‌سنجی فروسرخ تبدیل فوریه (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">FTIR</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">)، طیف‌سنجی پراش انرژی پرتوی ایکس (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">EDX</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">)، طیف‌سنجی فرابنفش مرئی (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">UV-visible</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) و آنالیز پتانسیل زتا (</span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">Zeta-potential</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">) برای شناسایی استفاده شد.<i> </i>زنده‌مانی سلول‌های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">ASPC-1</span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">و </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">Human Embyonic Kidnay 293 Cells</span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">(</span></span></span><em><span dir="LTR" style="font-size:10.0pt"><span style="color:black">HEK-293</span></span></em><em><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">)</span></span></span></em><em><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> بعد از تیمار با </span></span></span></em><span dir="LTR" style="font-size:10.0pt"><span style="color:black">Fe₃O₄@HA</span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">به‌روش </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">MTT</span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">بررسی شد. همچنین بیان‌ژن </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="color:black">CD44</span></span></i><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> در سلول‌های تیمارشده و کنترل</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">با تکنیک </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">qRT-PCR</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> تعیین شد</span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">. </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:14pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">یافته‌ها: </span></span></span></b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">کمپلکس </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">Fe₃O₄@HA</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> بر روی سلول‌های </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">ASPC-1</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> تأثیر قوی‌تری نسبت به </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">Fe₃O₄</span></span>_&nbsp;<span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">داشت</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> (05/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">p <</span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">)، درحالی‌که اثر </span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">آن</span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> بر روی سلول‌های سالم ضعیف‌تر بود (05/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">p ></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">). ضریب انتخاب دارو برابر 13/1 محاسبه شد.</span></span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتایج </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">qRT-PCR</span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نشان داد که بیان </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="color:black">CD44</span></span></i> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">در سلول‌های تیمارشده در غلظت </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">IC₅₀</span></span><span style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> 6،</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">درصد کمتر از </span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">سلول‌های </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">گروه </span></span></span><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">کنترل بود </span></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;(05/0 </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">p ></span></span><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">).</span></span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span></span><br> <span style="font-size:14pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتیجه</span></span></span></b><b><span dir="LTR" style="font-size:10.0pt"><span style="color:black">‌</span></span></b><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">گیری:</span></span></span></b> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">نتایج این پژوهش نشان داد که </span></span></span><span dir="LTR" style="font-size:10.0pt"><span style="color:black">Fe₃O₄@HA</span></span> <span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">اثرهای ضدتکثیریِ مناسبی مقابل سلول‌های سرطان لوزالمعده</span></span></span> <span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black">&nbsp;دارد و می‌تواند باعث کاهش بیان‌ژن </span></span></span><i><span dir="LTR" style="font-size:10.0pt"><span style="color:black">CD44</span></span></i><span lang="AR-BH" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"> شود.</span></span></span><b><span lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></b></span></span></span></span></span><br> &nbsp; <span style="font-size:14pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt">Aim</span></b> <b><span style="font-size:10.0pt">and Background</span></b><b><span style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.2pt">:</span></span></span></b><span style="font-size:10.0pt"><span style="color:black"> Pancreatic cancer is the third leading cause of cancer-related mortality. The utilization of hyaluronic acid in treatment is promising due to its targeted absorption and lack of adverse effects. The objective of this study was to examine the impact of iron oxide and hyaluronic nanoparticles on alterations in <i>CD44</i> gene expression within cancer cells. </span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span><br> <span style="font-size:14pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.2pt">Materials and Methods:</span></span></span></b> <span style="font-size:10.0pt"><span style="color:black">Iron oxide nanoparticles were synthesized using the co-precipitation method and then functionalized with hyaluronic acid in this study. Various confirmatory tests, such as Scanning Electron Microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR), Energy Dispersive X-Ray (EDX), Ultraviolet-Visible (UV-visible), and Zeta-potential, were conducted for characterization purposes. The viability of ASPC-1 and HEK-293 cells post-treatment with Fe3O4@HA was assessed using the MTT method. Additionally, the expression of the <i>CD44</i> gene in both treated and control cells was analyzed through the qRT-PCR technique. </span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span><br> <span style="font-size:14pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.2pt">Results:</span></span></span></b> <span style="font-size:10.0pt"><span style="color:black">The Fe3O4@HA complex exhibited a more potent impact on ASPC-1 cells compared to Fe3O4 (P</span></span> <span style="font-size:10.0pt"><span style="color:black"><</span></span> <span style="font-size:10.0pt"><span style="color:black">0.05), while its effect on normal cells was less pronounced (P</span></span> <span style="font-size:10.0pt"><span style="color:black">></span></span> <span style="font-size:10.0pt"><span style="color:black">0.05). The Selectivity Index (SI) for drug selection was determined to be 1.13. Analysis of qRTPCR data revealed a 6% decrease in <i>CD44</i> expression in treated cells at the IC₅₀ concentration, although this difference was not statistically significant (P</span></span> <span style="font-size:10.0pt"><span style="color:black">></span></span> <span style="font-size:10.0pt"><span style="color:black">0.05).</span></span><span dir="RTL" lang="FA" style="font-size:12.0pt"><span b="" nazanin="" style="font-family:"><span style="color:black"></span></span></span></span></span></span></span><br> <span style="font-size:14pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span new="" roman="" style="font-family:" times=""><b><span style="font-size:10.0pt"><span style="color:black"><span style="letter-spacing:-.2pt">Conclusion:</span></span></span></b><span style="font-size:10.0pt"><span style="color:black"> In conclusion, the outcomes of this investigation demonstrate that Fe3O4@HA possesses effective anti-proliferative characteristics against pancreatic cancer cells and has the ability to lower CD44 gene expression.</span></span><span lang="EN" style="font-size:10.0pt"><span style="color:black"></span></span></span></span></span></span><br> &nbsp; سرطان لوزالمعده, سلول‌های ASPC-1, ژن CD44, نانوذرات Fe3O4@HA, Pancreatic Cancer, ASPC-1 cells, CD44 gene, Fe3O4@HA nanoparticles. 73 86 http://ncmbjpiau.ir/browse.php?a_code=A-10-2000-2&slc_lang=other&sid=1 Seyedeh Motahareh Sadatfar سیده مطهره سادات فر 100319475328460015567 100319475328460015567 No MSc student, Department of Molecular-Cell Biology, Islamic Azad University, Lahijan Branch. Lahijan, Iran .گروه زیست‌شناسی- سلولی‌مولکولی، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران Khosro Issazadeh خسرو عیسی زاده 100319475328460015568 100319475328460015568 No Assistant Professor, Department of Microbiology, Faculty of Basic Sciences, Islamic Azad University, Lahijan Branch, Lahijan, Iran گروه میکروبیولوژی، دانشکدۀ علوم پایه، دانشگاه آزاد اسلامی، واحد لاهیجان، لاهیجان، ایران. Alireza Habibi علیرضا حبیبی alirh110@gmail.com 100319475328460015569 100319475328460015569 Yes Assistant Professor, Department of Basic Sciences, Science Higher Education Center, Imam Hossein (AS) Officer Training and Guards University, Tehran, Iran گروه علوم‌پایه، مرکز آموزش عالیِ علوم، دانشگاه امام حسین (ع)، تهران، ایران