fa مقایسه تاثیر نانو کلات آهن با کلات آهن بر پارامترهای رشد و فعالیت آنزیم های آنتی اکسیدان ریحان Ocimum Basilicum گیاه شناسی Botany مقاله پژوهشی Research Article <p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph"><font face="Times New Roman"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">سابقه و هدف: </span></b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">ریحان با نام علمی </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">Ocimum</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi"><span dir="rtl"></span> </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">Basilicum</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> گیاهی متعلق به راسته ی نعناع یان و تیره نعناع است،که به عنوان یک گیاه دارویی در طب سنتی ایران مورد استفاده قرارگرفته است. هدف از این پژوهش بررسی و مقایسه دو کود کلات آهن و نانو کود کلات آهن در غلظت های مختلف بر چند پاسخ فیزیولوژیکی و بیوشیمیایی گیاه ریحان است. در این تحقیق اثر نانو کود کلاته آهن و کود کلاته آهن بر پارامتر های رشد،<font size="2"><span style="mso-spacerun: yes">  </span>مقدار رنگیزه های فتوسنتزی برگ ، مقدار پروتئین و همچنین تغییرات فعالیت برخی آنزیم های آنتی اکسیدان در برگ بررسی شد.<p></p></font></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph"><font face="Times New Roman"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">مواد و روش ها:</span></b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"> آزمایش درشرایط کشت مزرعه ای، به صورت بلوک های کاملاً تصادفی با 4 تکرار طراحی شد. گیاهان در معرض غلظت های مختلفی از کود کلات آهن (^1-</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-bidi-language: FA" lang="AR-YE"> </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-bidi-language: FA" dir="ltr">kg</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-bidi-language: FA" lang="FA"><span dir="rtl"></span>.</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-bidi-language: FA" dir="ltr">ha</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-bidi-language: FA" lang="FA"><span dir="rtl"></span> 5/7 ، 5/4 ، 5/1</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">) و نانو کود کلات آهن (^1-</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">kg</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span>.</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">ha</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> 5، 3،1) قرار گرفتند.<p></p></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph"><font face="Times New Roman"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">یافته ها: </span></b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">بررسی ها نشان داد که پارامترهای رشد در گیاهانی که در معرض تیمار کود آهن با غلظت ^1- </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">kg</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span>.</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">ha</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> 5/7 و نانو کود آهن با غلظت ^1- </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">kg</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span>.</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">ha</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> 1 بودند، نسبت به شاهد افزایش یافته است. فعالیت آنزیم های آنتی اکسیدانی درحضور کود آهن در هر سه غلظت بیشتر از تیمارهای نانو کود آهن بود. همچنین نانو کود آهن سبب کاهش محتوای پروتئین در مقایسه با کود آهن گردید. افزایش در محتوای رنگیزه های فتوسنتزی در تیمار هایی که تحت نانو کود کلات آهن با غلظت ^1- </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">kg</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span>.</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">ha</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> 5 بودند، دیده شده است.<p></p></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph"><font face="Times New Roman"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">نتیجه گیری:</span></b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"> این نتایج نشان می دهد که جایگزینی کود آهن تهیه شده با فناوری نانو در مقایسه با کود های آهن رایج در غلظت مناسب یا کمتر نسبت به کود آهن می تواند سبب افزایش رشد کمی و کیفی گیاه ریحان شود.<p></p></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph"><font face="Times New Roman"><span style="LINE-HEIGHT: 120% LETTER-SPACING: 0.25pt FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"></span></font></p> <p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><font face="Times New Roman"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-ansi-language: CS" lang="CS">Aim and Background.</span></b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-ansi-language: CS" lang="CS"> Sweet basil (Ocimum basilicum) belongs to the Lamiaceae family. In this study the effect of nano iron chelated fertilizer and iron chelated fertilizer on growth parameters, leaves photosynthetic pigments, protein, and the activity of of some antioxidant enzymes in leaf were investigated.<p></p></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><font face="Times New Roman"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-ansi-language: CS" lang="CS">Materials and Methods.</span></b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-ansi-language: CS" lang="CS"> The experiment was conducted in farm condition in the form of randomized design based on four replications. Plants were treated by different concentrations of iron chelated fertilizer (1.5 ,4.5 ,7.5 kgha-1) and nano Fe chelated fertilizer (1, 3 ,5 kgha-1).<p></p></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><font face="Times New Roman"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-ansi-language: CS" lang="CS">Results.</span></b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-ansi-language: CS" lang="CS"> Results indicated that growth parameters increased in plants treated with Fe fertilizer (7.5 kgha-1) and nano Fe fertilizer with less than 5kgha-1. The antioxidant enzymes activity in the Fe<span dir="rtl"></span><span dir="rtl"><span dir="rtl"></span><font size="2"> </font></span>treatments was more than nano Fe treatments. Moreover, nano iron fertilizer resulted in the reduction of protein amounts in comparison with iron fertilizer treatments.<p></p></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph"><font face="Times New Roman"><b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-ansi-language: CS" lang="CS">Conclusion.</span></b><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi mso-ansi-language: CS" lang="CS">The results showed that Fe fertilizer could be replaced by nano Fe fertilizer.</span></font></p> Ocimum Basilicum، نانو کود کلات آهن، کود کلات آهن، پارامتر های رشد، رنگیزه های فتوسنتزی، آنزیم های آنتی اکسیدانی Ocimum basilicum, nano iron chelated fertilizer, iron chelated fertilizer, growth Parameters, photosynthetic pigments, antioxidant enzymes 89 98 http://ncmbjpiau.ir/browse.php?a_code=A-10-68-1&slc_lang=fa&sid=1 Maryam peyvandi مریم پیوندی m_peyvandi@iau-tnb.ac.ir 4569337333 1003194753284600655 Yes Department of Biology, Faculty of Biology Science, Islamic Azad University Tehran- North Branch دانشکده علوم زیستی دانشگاه آزاد اسلامی واحد تهران شمال Haniyeh Parande هانیه پرنده haniyeh_parandeh@yahoo.com 0075440210 1003194753284600656 No Department of Biology, Facoulty of Biology, Islamic Azad Univ, Tehran –North Branch دانشکده علوم زیستی دانشگاه آزاد اسلامی واحد تهران شمال Mahdi Mirza مهدی میرزا mirza@rifr.ac.ir 0938317237 1003194753284600657 No Department of Biology, Facoulty of Biology, Islamic Azad Univ, Tehran –North Branch بخش تحقیقات گیاهان داروئی و محصولات فرعی،موسسه جنگل ها و مراتع کشور