fa تشخیص موتاسیون مقاوم به دارو ناحیه YMDD ژن پلی مراز ویروس HBV دربیماران مبتلا به هپاتیت B از طریق روش ( Ligase Chain Reaction (LCR سلولی و مولکولی Cellular and molecular مقاله پژوهشی Research Article <p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><font size="3"><span><strong>سابقه و هدف:</strong> هپاتیت </span><span style="LETTER-SPACING: 0.1pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">B</span><span dir="rtl"></span><span style="LETTER-SPACING: 0.1pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span>، بیماری بسیار خطرناکی است که به صورت اندمیک در بسیاری از مناطق جهان مشاهده می شود.</span></font></font><font face="times new roman,times,serif"><font size="3"><span style="LETTER-SPACING: 0.1pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">ویروس هپاتیت<span style="mso-spacerun: yes">  </span></span><span style="LINE-HEIGHT: 120% LETTER-SPACING: 0.1pt FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span style="mso-spacerun: yes"> </span></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">(</span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">HBV</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span>)</span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"> نسبت به یک سری داروهای ضد ویروسی از جمله لامیوودین حساس بوده (%98)، بطوری که به نواحی خاصی از آنزیم </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">DNA</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> پلی مراز ویروس متصل می گردد و مانع عمل ترانس کریپتاز معکوس و همانند سازی </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">DNA</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> ویروسی </span></font></font><font face="times new roman,times,serif"><font size="3"><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">می شود. در اثر مصرف طولانی مدت دارو، جهشی در موتیف </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">YMDD</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> در دومین </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">C</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> ژن پلیمراز ویروس ایجاد می شود که سبب مقاومت ویروس نسبت به این دارو می گردد. در این مطالعه به منظور شناسایی سریع تر و دقیق تر موتاسیون </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">YMDD</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> و بنابر اهمیت شیوع موتاسیون فوق، جهت مقاومت ویروس نسبت به دارو، از طرفی ارتباط بین این تغییر ژنومی با پیشرفت بیماری های کبدی و اثر آن بر روی سیر بیماری زایی و پاسخ به درمان، روش </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">LCR</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> مورد استفاده قرار گرفت.<p></p></span></font></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><font size="3"><span><strong>مواد و روش ها:</strong> در این مطالعه، 62 نمونه حاصل از </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">Nested</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi"><span dir="rtl"></span> </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">PCR</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> ژن پلی مراز ویروس </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">HBV</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> از طریق تکنیک </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">LCR</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> مورد </span></font></font><font face="times new roman,times,serif"><font size="3"><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">بررسی قرار گرفت. سه جفت الیگونوکلئوتید </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">bp</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi"><span dir="rtl"></span> </span><span>26-21 </span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">برای ناحیه </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">YMDD</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> هم برای نمونه نرمال و هم برای نمونه موتان طراحی گردیدکه سبب تکثیر ناحیه </span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">bp</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> 47 شد.<p></p></span></font></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><font size="3"><span><strong>یافته ها:</strong> از 62 نمونه مورد بررسی، 51 نفر (25/82%)، بدون موتاسیون در ناحیه </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">YMDD</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> و 11 بیمار (74/17%) دارای موتاسیون در ناحیه فوق بودند.<p></p></span></font></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><font size="3"><span><strong>نتیجه گیری:</strong> بررسی نتایج حاصل از </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">LCR</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> نشان دادکه در بیماران هم نوع وحشی و هم نوع موتان </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">HBV</span><span dir="rtl"></span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi"><span dir="rtl"></span> </span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE">وجود دارد در نتیجه برای بررسی وجود یا عدم وجود موتاسیون از الیگونوکلئوتیدهای موتان استفاده شد. نتایج حاصل از روش </span><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr">LCR</span><span dir="rtl"></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" lang="AR-YE"><span dir="rtl"></span> مطابقت کامل با نتایج حاصل از تعیین توالی نشان داد.<p></p></span></font></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt" dir="rtl" class="BasicParagraph" align="justify"><span style="LINE-HEIGHT: 120% FONT-SIZE: 10pt mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="ltr"><p><font size="3" face="times new roman,times,serif"> </font></p></span></p> <p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><span><font size="2"><strong>Aim and background.</strong> Hepatitis B and its incidence are considered as an endemic disease worldwide. In addition to creation of critical and chronic hepatitis B disease, in patients who suffer from chronic kind of this infection there are hepatocelullar carcinoma or hepatic cirrhosis presumptions or other kinds of defections. HBV (hepatitis B virus) is also up to 98 <span style="LETTER-SPACING: -0.35pt">percent sensitive to some medicines like lamivudine. Lamivudine blocks viral DNA polymera</span>se enzyme reverse transcriptase action through binding to its specific regions. But problem may be formed in patients whom are exposed to lamivudine for long time a new mutation occurs in C domain of YMDD motif and of viral DNA polymerase gene that leads to lamivudine resistance. Molecular diagnosis of this mutation is essential with regard to considerable relationship between mutation occurrence rate and infection incidence range due to host immune system. We chose LCR method due to its ease achievability compared with others to detect the mutation. <p></p></font></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><span><font size="2"><strong>Materials and methods.</strong> 62 Nested PCR viral polymerase gene samples of HBV using LCR were surveyed. Five pairs of 21_26bps oligonucleotides were designed for both wild type and mutant YMDD region which amplify a 47bps region.<p></p></font></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><span><font size="2"><strong>Results.</strong> 51 of 62 patients have been non mutant in YMDD motif (82.25%) while 11 patients have been mutant in YMDD motif (17.74%). LCR. <p></p></font></span></font></p><p style="TEXT-ALIGN: justify MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><span><font size="2"><strong>Conclusion</strong>. Investigation of LCR conclusions has shown that there is both wild and mutant type of HBV in patients. For this reason, we utilized mutant oligonucleotides and its bonds on gel were seen. Results are completely in conformation to sequencing conclusions.<p></p></font></span></font></p><p style="TEXT-ALIGN: left MARGIN: 0in 0in 0pt unicode-bidi: embed DIRECTION: ltr" class="BasicParagraph" align="justify"><font face="times new roman,times,serif"><font size="2"><span></span><span style="mso-ascii-font-family: 'Times New Roman' mso-ascii-theme-font: major-bidi mso-hansi-font-family: 'Times New Roman' mso-hansi-theme-font: major-bidi mso-bidi-font-family: 'Times New Roman' mso-bidi-theme-font: major-bidi" dir="rtl" lang="AR-YE"><p></p></span></font></font></p> هپاتیت B، ژن پلی مراز ویروس هپاتیت B، موتاسیون YMDD، روش LCR Hepatitis B, viral DNA polymerase gene, YMDD mutation, LCR method 27 35 http://ncmbjpiau.ir/browse.php?a_code=A-10-69-2&slc_lang=fa&sid=1 Leyla Khojareh لیلا خجاره l_khojareh@yahoo.com 1003194753284600639 1003194753284600639 Yes Tarbiat Modares University دانشگاه تربیت مدرس Mgid Sadeghizadeh مجید صادقی زاده 1003194753284600640 1003194753284600640 No Tarbiat Modares University دانشگاه تربیت مدرس Golnaz Bahramali گلناز بهرامعلی 1003194753284600641 1003194753284600641 No Tarbiat Modares University دانشگاه تربیت مدرس Saman Hosseinkhani سامان حسینخانی 1003194753284600642 1003194753284600642 No Tarbiat Modares University دانشگاه تربیت مدرس Mehrdad Behmanesh مهرداد بهمنش 1003194753284600643 1003194753284600643 No Tarbiat Modares University دانشگاه تربیت مدرس