fa بررسی اثر آنتیاکسیدانی و سیتوتوکسیک عصاره اتانولی برگ گیاه گزنه(Urtica dioica L.) بر روی رده سلولی A549 ژنتیک Genetics مقاله پژوهشی Research Article <p dir="RTL" style="text-align:justify;line-height:normal;direction:rtl;unicode-bidi:embed;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سرطان ریه مهم</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ترین عامل مرگ ناشی از سرطان در دنیا می</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">باشد و از آن جایی که اغلب درمان</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های امروزی سرطان ناکارآمد و دارای عوارض جانبی زیادی هستند، لذا یافتن درمان موثر و جایگزین ضروری به نظر می</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">رسد. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گیاه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گزنه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نام</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">علمی </span></span><em><span dir="LTR">Urtica dioica </span></em><span dir="LTR">L.</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تیره </span></span><em><span dir="LTR">Urticaceae</span></em> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گیاهی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">چند</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ساله، علفی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گلدار</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">است</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">که</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ارزش</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">غذایی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دارویی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بالایی دارد. لذا</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در پژوهش</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حاضر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تاثیر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سیتوتوکسیکی عصاره اتانولی برگ گیاه گزنه </span></span><span dir="LTR">(<em>Urtica dioica </em>L.)</span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روی رده ی سلولی</span></span> <span dir="LTR">A549</span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مورد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بررسی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">قرار</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گرفت</span></span><span dir="LTR"><span style="font-size:12.0pt;">.</span></span></p> <p dir="RTL" style="text-align:justify;line-height:normal;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">مواد و روش ها:</span></span></strong></p> <p dir="RTL" style="text-align:justify;line-height:normal;direction:rtl;unicode-bidi:embed;">&nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در این مطالعه،</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">برای</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اولین</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بار، اثر سیتوتوکسیکی عصاره اتانولی برگ گیاه گزنه بر روی رده ی سلولی </span></span><span dir="LTR">A549</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> مورد بررسی قرار گرفت. به طوری که برگ</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گیاه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گزنه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دوپایه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اردیبهشت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ماه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سال</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">1394</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">منطقه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سه</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هزار تنکابن</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جمع</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آوری</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شد و جهت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بررسی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">فعالیت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آنتی</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اکسیدانی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش</span></span> <span dir="LTR">DPPH⁰</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مورد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ارزیابی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">قرار</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گرفت.</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">رده</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سلولی </span></span><span dir="LTR">A549</span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">محیط </span></span><span dir="LTR">DMEM</span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با 20 درصد سرم </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">جنین</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گاوی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کشت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داده</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شد</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">.</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">غلظت</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مختلف عصاره</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اتانولی برگ</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گیاه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گزنه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دو</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">پایه (5، 5/2، 25/1 و 5/0 میلی</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گرم بر میلی</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">لیتر) به مدت 24، 48 و 72 ساعت بر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سلول</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سرطانی</span></span> <span dir="LTR">A549</span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اثر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داده</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شد.</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ادامه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">توانایی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حیات</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سلول</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ها</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">با استفاده</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روش </span></span><span dir="LTR">MTT</span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بررسی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شد</span></span><span dir="LTR"><span style="font-size:12.0pt;">.</span></span></p> <p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;line-height:normal;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">یافته</span></span></strong><strong><span dir="LTR">‌</span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">ها:</span></span></strong></p> <p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;line-height:normal;direction:rtl;unicode-bidi:embed;"><span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در</span></span></span> <span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بررسی</span></span></span> <span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حاضر درصد مهار رادیکال آزاد </span></span></span><span dir="LTR"><span style="color:red;">DPPH⁰</span></span><span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:14.0pt;"> برابر با</span></span></span><span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> 79/67 درصد و همچنین مقدار </span></span></span><span dir="LTR"><span style="color:red;"><span style="font-size:12.0pt;">IC₅₀ </span></span></span><span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">30/4 میکروگرم برمیلی</span></span></span><span dir="LTR"><span style="color:red;"><span style="font-size:12.0pt;">‌</span></span></span><span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">لیتر</span></span></span> <span style="color:red;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">محاسبه گردید. </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">حاصل</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ازتست</span></span> <span dir="LTR">MTT</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ، </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این پژوهش نشان</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">که</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عصاره اتانولی برگ گیاه گزنه اثر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سایتوتوکسیک</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">وابسته</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دوز</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بر روی رده ی سلولی </span></span><span dir="LTR">A549</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دارد. </span></span><span dir="LTR">IC₅₀</span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این عصاره</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">برای</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سلول</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span dir="LTR">A549</span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">غلظت 5 </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">می</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">لی‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گرم</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">میلی</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">لیتر </span></span>&nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به دست</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آمد.</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج </span></span><span dir="LTR">MTT</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مدت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">زمان</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">48 و 72 ساعت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تفاوت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">معنی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داری</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> نداشتند، ولی با مدت زمان 24 ساعت تفاوت معنی</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دار بود.</span></span></p> <p dir="RTL" style="text-align:justify;line-height:normal;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">نتیجه</span></span></strong><strong><span dir="LTR">‌</span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">گیری: </span></span></strong></p> <p dir="RTL" style="text-align:justify;line-height:normal;direction:rtl;unicode-bidi:embed;"><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">عصاره اتانولی برگ گیاه گزنه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">صورت</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">وابسته</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دوز</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">قادر، </span></span>&nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بین</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بردن</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سلول</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سرطانی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بوده</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">خواص</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سایتوتوکسیکی از خود نشان داد.</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;بدین ترتیب این گیاه می تواند به عنوان یک گیاه دارویی بر علیه سرطان ریه مورد پژوهش وسیع</span></span><span dir="LTR"><span style="font-size:12.0pt;">‌</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تری قرار گیرد.</span></span>&nbsp;</p> Aim and Background: Among cancers, Lung cancer is considered to be the most important cause of death in worldwide and as the most modern cancer treatments are ineffective with side effects, it seems essential to find impressive alternatives treatment. Nettle plant with the scientific name Urtica dioica L. Of family Urticaceae, is a flowering and Perennial plant that has high nutritional and medicinal value. There fore, the cytotoxic effect of ethanolic extract of nettle leaf (Urtica dioica L.) on A549 cell lines was evaluated. Materials and methods: In this study, for the first time, the cytotoxic effect of ethanolic extract of nettle leaves on A549 cell lines was evaluated. The dioecious nettle leaves in April 2015 of sehezare Tonekabon were collected and studied; Then the antioxidant activity was evaluated by DPPH0 Assay. A549 cell line in DMEM with 20% fetal bovine serum was grown. Different concentrations of ethanolic extract of two legs nettle leaves (5, 2/5, 1/25 and 0/5 mg/ml) for 24, 48 and 72 hours were applied on cancer cells A549. Next the viability of cells using MTT assay was considered. Results: In this study, the percent of free radical scavenging DPPH0 equals to 67/79 percent and the amount of IC50 was calculated to be 4/30 mg/ml. The results of MTT assay showed that the ethanolic extract of nettle leaves has a cytotoxic effect on the A549 cell line which is dependent to their doses. Also IC50 of the extract concentration for A549 cells was obtained to be 5 mg/ml. MTT results showed no significant differenc in 48 and 72 hours. But the difference was significant at 24 hours. Conclusion: The ethanolic extract of nettle leaves which is dose-dependent could destroy the cancer cells and showed cytotoxic properties. Thus, this plant can be used as a medicinal plant against lung cancer in wider scope. Urtica dioica L., سرطان, A549, عصاره اتانولی, سیتوتوکسیک, آنتیاکسیدانی Urtica dioica L., cancer, A549, ethanolic extract, cytotoxic, Antioxidant 27 34 http://ncmbjpiau.ir/browse.php?a_code=A-10-995-1&slc_lang=fa&sid=1 Sanaz nikoumanesh ساناز نیکومنش 10031947532846007225 10031947532846007225 No Department of Cell and Molecular Biology, College Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran گروه زیست شناسی سلولی و مولکلولی، دانشکده علوم زیستی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران Ali Mohammad Asgharian علی محمد اصغریان mehranasgharian@yahoo.com 10031947532846007226 10031947532846007226 Yes Department of Cell and Molecular Biology, College Biology, Tonekabon Branch, Islamic Azad University, Tonekabon, Iran گروه زیست شناسی سلولی و مولکلولی، دانشکده علوم زیستی، واحد تنکابن، دانشگاه آزاد اسلامی، تنکابن، ایران