fa بررسی اثرات ضدتکثیری و القاء آپوپتوز کمپلکس‌های نیکل با لیگاندهای مالتول، دیفریپرون و 3-هیدروکسی فلاون بر روی چند رده‌‌ی سلول سرطانی ژنتیک Genetics مقاله پژوهشی Research Article <p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;text-justify:kashida;text-kashida:0%;line-height:normal;background:white;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">سابقه و هدف:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعات</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نشان</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">داده&shy;اند که</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">برخی لیگاندها نظیر مالتول با کئوردینه&shy;شدن با فلزات،</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">موجب القاء</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آپوپتوز و مهار تکثیر سلول&shy;های سرطانی می&shy;شود. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هدف</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">مطالعه</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">،</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> سنتز </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کمپلکس</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;های</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> جدیدی از نیکل و</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ارزیابی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اثر</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ات</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آپوپتیک و ضدتکثیری</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ترکیب</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ات</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">می&shy;باشد.</span></span></p> <p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;line-height:normal;background:white;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">مواد و روش&shy;ها: </span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در این مطالعه،</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کمپلکس&shy;هایی از نیکل و لیگاندهای مالتول، دیفریپرون و 3-هیدروکسی&shy;فلاون</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سنتز&shy;گردید. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سایتوتوکسیسیته</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این کمپلکس&shy;ها به منظور ارزیابی فعالیت ضد&shy;سرطانی آن</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روی</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">رده&shy;های </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">HeLa </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">MCF-7</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">MDA-MB-231</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">NALM-6</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">Neuro-2a</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">L929</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با روش</span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">MTT </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&nbsp;و فلوسایتومتری، بررسی&shy;گردید. </span></span></p> <p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;line-height:normal;background:white;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">یافته&shy; ها:</span></span></strong> <span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">IC₅₀ &plusmn; SD (&mu;M) </span></span></span>&nbsp;&nbsp;<span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به&shy;دست</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آمده</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">از</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اثر کمپلکس</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">&shy;های</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">1 تا 3 بر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روی رده&shy;های سلولی</span></span> <span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">HeLa </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">MCF-7</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">MDA-MB-231</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">NALM-6</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">Neuro-2a</span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">L929</span></span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به ترتیب در گستره&shy;ی 6/15</span></span> <span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">تا</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">1/199، 00/5</span></span> <span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">تا</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">2/185 و&nbsp; 8/39</span></span> <span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">تا</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">8/168، می&shy;باشند. </span></span></p> <p dir="RTL" style="margin-bottom:0cm;margin-bottom:.0001pt;text-align:justify;line-height:normal;background:white;direction:rtl;unicode-bidi:embed;"><strong><span style="font-family:b nazanin;"><span style="font-size:14.0pt;">نتیجه &shy;گیری: </span></span></strong><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج به&shy;دست&shy;آمده</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سایتوتوکسیسیته&shy;ی بیشتری را برای کمپلکس&shy;ها در مقابل رده&shy;های سلولی </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">MDA-MB-231</span></span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و </span></span><span dir="LTR"><span style="font-family:blotus;"><span style="font-size:10.0pt;">Neuro-2a</span></span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نشان داد. </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به دست آمده اثبات می&shy;&shy;کند کمپلکس سنتز شده هیچگونه</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تأثیر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">سایتوتوکسیکی بر</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روی بر سلول&shy;های نرمال فیبروبلاست موش</span></span><span dir="LTR"><span style="font-family:bnazanin;"><span style="font-size:12.0pt;"> (</span></span></span><span dir="LTR"><span style="font-family:times new roman,serif;"><span style="font-size:10.0pt;">L929</span></span></span><span dir="LTR"><span style="font-family:bnazanin;"><span style="font-size:12.0pt;">) </span></span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ندارد</span></span><span dir="LTR"><span style="font-family:bnazanin;"><span style="font-size:12.0pt;">.</span></span></span></p> Aim and Background: Studies demonstrated that some ligands such as maltol in coordination with metals, induce apoptosis and inhibit the cancer cells proliferation. The aim of this study is the synthesis of new complexes of nickel and evaluation of anti-proliferative and apoptotic effects of these compounds. Material and methods: In this study, we synthesized the novel complexes of nickel with maltol, deferiprone and 3-hydroxyflavone ligands. The cytotoxicity of this complex was screened for antitumor activity against HeLa, MCF-7, MDA-MB-231, NALM-6, Neuro-2a and L929 by MTT assay and Flow cytometry. Results: The IC50 ± SD (μM) values, calculated for synthesized complex against HeLa, MCF-7, MDA-MB-231, NALM-6, Neuro-2a and L929 cell lines are between 15.6 to 199.1, 5.00 to 185.2 and 39.8 to 168.8 for complexes 1 to 3 respectively. Conclusion: Our results showed that investigated complex exhibited higher cytotoxic activities against MDA-MB-231 and Neuro-2a compared to other cell lines. Our results confirmed that the synthesized compound have not any cytotoxicity effects on mouse fibroblast cell line (L929). نیکل, مالتول, دیفریپرون, 3-هیدروکسی فلاون, روشMTT , فلوسایتومتری, L929. Nickel, Maltol, Deferiprone, 3-hydroxyflavone, MTT assay, Flow cytometry, L929 43 58 http://ncmbjpiau.ir/browse.php?a_code=A-10-780-5&slc_lang=fa&sid=1 Shamila Azmoodeh شمیلا آزموده 10031947532846007232 10031947532846007232 No Department of Chemistry, University of Mashhad, Mashhad, Iran گروه شیمی، دانشگاه فردوسی مشهد، مشهد، ایران Mohammad Izadyar محمد ایزدیار 10031947532846007233 10031947532846007233 No Department of Chemistry, University of Mashhad, Mashhad, Iran گروه شیمی، دانشگاه فردوسی مشهد، مشهد، ایران Amir Shokooh Saljooghi امیر شکوه‌سلجوقی saljooghi@um.ac.ir 10031947532846007234 10031947532846007234 Yes Department of Chemistry, University of Mashhad, Mashhad, Iran گروه شیمی، دانشگاه فردوسی مشهد، مشهد، ایران Maryam babaee مریم بابایی 10031947532846007235 10031947532846007235 No Department of Chemistry, University of Mashhad, Mashhad, Iran گروه شیمی، دانشگاه فردوسی مشهد، مشهد، ایران