fa تداخل سیستم های اندوکانابینوییدی ، کولینرژیک و توام آنها بر حا فظه موش ژنتیک Genetics مقاله پژوهشی Research Article <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">سابقه و هدف</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">کانابینوییدها به عنوان ضعیف کننده حافظه در انسان و حیوانات شناخته شده </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اند که</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> از طریق </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گیرنده های</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> خود </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">تاثیر</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گذارند.</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">این ترکیب ها</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> با اثر بر هیپوکامپ می توانند</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بر</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> حافظه و یادگیری اثر گذار باشند</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">استیل کولین به عنوان یک انتقال دهنده عصبی، نقش اساسی طی فرایند یادگیری و حافظه ایفا می کند و دونپزیل</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">می تواند درسیناپس های کولینرژیک اثر تقویت کننده بر این ماده داشته باشد. در این پژوهش اثر دونپزیل, </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">URB597</span></span></em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> &quot;تقویت کننده سیستم اندوکانابینوییدی&quot; و اثر توام </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">هر دو ماده</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بر حافظه موش مورد بررسی قرار گرفت.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">مواد و </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">روش</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;"> ها</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در این پژوهش تجربی </span></span><span style="font-family:b nazanin;">40</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> سر موش سوری با محدوده وزنی </span></span><span style="font-family:b nazanin;">35-20 </span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">گرم به گروه های کنتر</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ل</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">، </span></span><span dir="LTR"><span style="font-size:10.0pt;">URB597)</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">)</span></span> <span dir="LTR"><span style="font-size:10.0pt;">mg/kg,ip)</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">1/0</span></span> <span dir="LTR"><span style="font-size:10.0pt;">(</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> دونپزیل</span></span><span dir="LTR"><span style="font-size:10.0pt;">mg/kg,ip)</span></span> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">5/0</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">) </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و گروه </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ترکیبی</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دونپزیل </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span> <em><span dir="LTR"><span style="font-size:10.0pt;">URB597</span></span></em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> تقسیم شدند. روز اول </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">به </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">موش ها در دستگاه شاتل باکس، شوک الکتریکی </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">وارد شد</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">در </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">روز دوم </span></span><span style="font-family:b nazanin;">15</span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دقیقه قبل از تست حافظه تزریق مواد به صورت درون صفاقی صورت گرفت. پارامترهای مورد سنجش، اولین زمان ورود به بخش </span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">تاریک</span></span><span dir="LTR"><span style="font-size:10.0pt;">STLr)</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">) </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و مجموع زمان ماندن در آن بخش(</span></span><span dir="LTR"><span style="font-size:10.0pt;">TDC</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">) </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">بود. </span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">یافته ها:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آنالیز داده ها اختلاف معناداری در</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دریافت تعداد</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">شوک ها نشان نداد، اما مقایسه </span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">میزان</span></span><em><span dir="LTR"><span style="font-size:10.0pt;">STLr</span></span></em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بین گروه </span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ها</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ی </span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">کنتر</span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">ل, </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">URB597</span></span></em> <span style="font-family:b nazanin;"><span style="font-size:10.0pt;">(</span></span><span dir="LTR"><span style="font-size:10.0pt;">(P<0.001</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ترکیب </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دونپزیل</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و</span></span> <em><span dir="LTR"><span style="font-size:10.0pt;">URB597</span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;"> ( </span></span><span dir="LTR"><span style="font-size:10.0pt;">(P<0.001</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و نیز در میزان </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">TDC</span></span></em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بین گروه </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">URB597</span></span></em> <span dir="LTR"><span style="font-size:10.0pt;">(P<0.001)</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> در مقایسه با کنترل</span></span><span dir="LTR"><span style="font-size:10.0pt;">P<0.001) </span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">)</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> ترکیب</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> دونپزیل </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">و</span></span><em><span dir="LTR"><span style="font-size:10.0pt;">URB597 </span></span></em>&nbsp;<span dir="LTR"><span style="font-size:10.0pt;">(P<0.005)</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اختلاف معنا داری مشاهده گردید </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">اضافه</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> بر این,گروه </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">ترکیبی </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">دونپزیل</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> و </span></span><em><span dir="LTR"><span style="font-size:10.0pt;">URB597</span></span></em><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">(</span></span><span dir="LTR"><span style="font-size:10.0pt;">(P<0.005</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">درمقایسه با گروه کنترل اختلاف معناداری را نشان دا</span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">د.</span></span><br> <strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">نتیجه</span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;"> گیری </span></span></strong><strong><span style="font-family:b nazanin;"><span style="font-size:16.0pt;">:</span></span></strong> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نتایج</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">آزمایش ها</span></span> <span style="font-family:b nazanin;"><span style="font-size:12.0pt;">نشان داد </span></span><span style="font-family:b nazanin;"><span style="font-size:10.0pt;">که</span></span><em><span dir="LTR"><span style="font-size:10.0pt;">URB597</span></span></em><span style="font-family:b nazanin;"><span style="font-size:12.0pt;"> موجب تخریب حافظه شده و تیمار دونپزیل &quot;تقویت کننده سیستم کولینرژیک&quot; اثر بهبود دهنده روی حافظه تخریب شده داشته و این موضوع تداخل عمل بین دو سیستم نروترانسمیتری را نشان می دهد. که موجب تقویت حافظه شده است </span></span><span style="font-family:b nazanin;"><span style="font-size:12.0pt;">.</span></span><br> &nbsp; Aim and Background: Cannabinoids are known to attenuate memory in both humans and animals that effects of this compounds act through their receptors.These compounds can affect on the memory and learning through the hippocampus. Acetylcholine, as a neurotransmitter, plays fundamental role during process of learning and memory and donepezil can be potentiation effect in cholinergic synapse On this material. In this research the effects of donepezil, URB597 “potentiation endocannabinoid system " and effect of both them on the memory in mice was investigated. Materials and Methods: In this experimental research 40 male mice weighting 20-35 g were divided to the 4 groups including control, URB597 (0.1 mg/kg, i.p), donepezil (0.5 mg/kg, i.p.) and URB597 & donepezil. Mice to the first day received in the Shuttle box electrical trial then at the second day intraperitoneal administrate of drugs 15 minutes before the retrieval test. Two analysis parameters, delay the first time in the dark chamber (STLr) and sum of to remain time in the dark chamber (TDC). Result: Analysis of data indicated, that there was not significant difference in received number of trials. But there was significant difference between groups control and URB597 (p<0.001) in STLr. Also there was significant difference URB597 (p<0.001) in comparison by control and compound URB597 & donepezil groups in TDC and also there was significant difference in compound URB597 & donepezil (p<0.005) group in comparison to control group. Conclusion: Result of this experiment shown that URB597 led to memory impairment and administration of donepezil, as cholinergic system potentiation, has improvement affect on the destructitive memory, so indicate interference of both system led to memory reinforcement. کانابینویید, سیستم کولینرژیک, حافظه , موش سوری Cannabinoids, Cholinergic system, Memory, Mice 25 32 http://ncmbjpiau.ir/browse.php?a_code=A-10-675-2&slc_lang=fa&sid=1 Siamak Shahidi سیامک شهیدی 10031947532846007465 10031947532846007465 No Department of Physiology, Hamedan University of Medical Sciences, Hamedan, Iran دانشگاه علوم پزشکی همدان, همدان ایران Tayebe Tousan طیبه توسن nastaranshad74@yahoo.com 10031947532846007466 10031947532846007466 No Department of Biology, Islamic Azad University of Hamedan Branch, Hamedan, Iran گروه زیست شناسی, دانشکده علوم پایه, دانشگاه آزاد اسلامی واحد همدان, همدان, ایران Fateme Kazemeini فاطمه کاظمینی 10031947532846007467 10031947532846007467 Yes Department of Biology, Islamic Azad University of Broujerd Branch, Broujerd, Iran, گروه زیست شناسی, دانشکده علوم پایه, دانشگاه آزاد اسلامی واحد بروجرد, بروجرد, ایران