Volume 8, Issue 30 (4-2018)                   NCMBJ 2018, 8(30): 110-123 | Back to browse issues page

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Department of Microbiology, Collage of science, North Tehran Branch, Islamic Azad University, Tehran, Iran , emi_biotech2006@yahoo.ca
Abstract:   (5383 Views)
Aim and Background: Male urogenital tract infection is one of the most causes of male infertility. Among bacterial species, E.coli is the most important pathogen thatcaused defection in spermatogenesis. The purpose of this study was todetermine of fim H,pap and afa genes in E.coli isolated of infertile men semen.
Materials and Methods: 100 semen samples were cultured. Bacteria were identified according to standard bacteriological methods. Then DNA was extracted from E.coli  isolated and  frequency of afa,pap and fim H genes were analyzed by PCR.
Results: from 100 samples of semen,53 bacteria were isolated, as, E.coli (18.86%), staphylococci (33.33%), streptococci (45/09%) and klebsiella (5/88%). E.coli infected samples had abnormality in sperm motility and morphology.  Frequency of fim H and pap genes in E.coli strains were 100%, also afa gene didn`t find in any of samples.
Discussion: results showed that 18.86% E.coli was isolated from infertile men semens. E. coli is an important genital pathogen and prevalence of the pathogenic genes, including adhesions, is effective in the control and treatment of the male infertility. This study indicated the prevalence of  fim H and pap genes in E.coli contaminated samples could be related to males infertility.
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Type of Study: Research Article | Subject: Microbiology
Received: 2018/04/25 | Accepted: 2018/04/25 | Published: 2018/04/25

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