Volume 11, Issue 43 (7-2021)
NCMBJ 2021, 11(43): 91-100 |
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Yaghobi moghaddam M, Saeedinia A, Samimi Nemati A, Dehghan M. Cloning, expression and purification of vibrioblock proteins as candidates for cholera. NCMBJ 2021; 11 (43) :91-100
URL: http://ncmbjpiau.ir/article-1-1394-en.html
URL: http://ncmbjpiau.ir/article-1-1394-en.html
Faculty of Chemistry and Chemical Engineering, Malek Ashtar University of Technology, Iran
Abstract: (2078 Views)
Aim and Background: With the advent of cloning technology and recombinant DNA, it became possible to produce drugs and vaccines that were not possible in the past. Recombinant protein occupies a large part of the biopharmaceutical industry, one of the most important of which is vaccination.
Material & Methods: In this study, with the aim of obtaining a protein candidate for cholera vaccine, first cloning of a plasmid designed for the bacterial host E.coli DE3 Rossetagami was transformed, then expression was induced using IPTG and after protein expression was examined by SDS-PAGE gel electrophoresis, and then the protein was purified by nickel-sepharose (NI-NTA) chromatography column.
Result:The results show that the purified protein has a molecular weight of 31 kDa and the amount of purified protein is 1 mg.
Conclusion:Based on previous studies as well as the results of this study, the resulting protein can be considered as a suitable candidate for the cholera vaccine.
Type of Study: Research Article |
Subject:
Cellular and molecular
Received: 2020/12/15 | Accepted: 2021/06/20 | Published: 2021/07/1
Received: 2020/12/15 | Accepted: 2021/06/20 | Published: 2021/07/1
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