Volume 11, Issue 43 (7-2021)                   NCMBJ 2021, 11(43): 46-62 | Back to browse issues page

XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Ghashghaei M, Akhlaghi M. Investigation of nanoniosomal formulation containing doxorubicin effect on ovarian cancer cell line (OVCAR-3 cell line). NCMBJ 2021; 11 (43) :46-62
URL: http://ncmbjpiau.ir/article-1-1395-en.html
2. Department of Biochemistry, Islamic Azad University Branch Shiraz, Shiraz, Iran
Abstract:   (2561 Views)
Aim & Background: Cancer is a major cause of mortality in the world. Treatment of Cancer through drug therapy/chemotherapy faces the challenges of drug delivery. Drug delivery systems such as niosomes provided high efficiency and targeted treatment. The aim of this study was to prepare a new formula of niosomal nano system containing doxorubicin as the cancer therapy drug. Changing the tissue distribution of drug leads to drug effect improvement and reducing its cytotoxicity.
 
Materials & Methods: Doxorubicin encapsulated niosomes were synthesized using thin-film method. A certain amount of Span-60 and Cholesterol dissolved by chloroform in a round bottom balloon. Rotary evaporator was used in order to remove the organic solvent and form the thin-film. The thin-film was hydrated by doxorubicin and PBS solution using rotary evaporator in 10°C higher than span-60 transition phase. Entrapment Efficiency and release profile were investigated using dialysis and spectrophotometry. Size reduction was applied by probe sonicating. Nanoparticles average diameter and zeta potentiol was measured using DLS technique and Zeta sizer. The optimum formula was PEGylated and its cytotoxicity investigated through MTT assay on Ovarian cancer cell line and fibroblast normal cells.
 
Results: The diameter of optimized and PEGylated niosomal formulation was 172.5 nm and entrapment efficiency of PEGylated doxorubicin system was about 95.83±1.18. release studies result in PBS buffer at 37°C was the sign of PEGylated nanoniosomes efficiency in controlling the drug release, which the release percent of drug after 48 hours was 20.52 and its cytotoxicity was higher than the free drug.
 
Conclusion: The study demonstrates using of drug delivery carriers such as synthesized nano-niosomes has an effective role in increasing the efficacy and reducing the consumed dosages of drug.
 
Full-Text [PDF 651 kb]   (826 Downloads)    
Type of Study: Research Article | Subject: Cellular and molecular
Received: 2021/04/28 | Accepted: 2021/06/23 | Published: 2021/07/1

Add your comments about this article : Your username or Email:
CAPTCHA

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | New Cellular and Molecular Biotechnology Journal

Designed & Developed by : Yektaweb